eNOS is required for acute in vivo ischemic preconditioning of the heart: Effects of ischemic duration and sex

M. A Hassan Talukder, Fuchun Yang, Hiroaki Shimokawa, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

Ischemic preconditioning (IPC) is a powerful phenomenon that provides potent cardioprotection in mammalian hearts; however, the role of endothelial nitric oxide (NO) synthase (eNOS)-mediated NO in this process remains highly controversial. Questions also remain regarding this pathway as a function of sex and ischemic duration. Therefore, we performed extensive experiments in wild-type (WT) and eNOS knockout (eNOS-/-) mice to evaluate whether the infarct-limiting effect of IPC depends on eNOS, ischemic periods, and sex. Classical IPC was induced by three cycles of 5 min of regional coronary ischemia separated by 5 min of reperfusion and was followed by 30 or 60 min of sustained ischemia and 24 h of reperfusion. The control ischemia-reperfusion protocol had 30 or 60 min of ischemia followed by 24 h of reperfusion. Protection was evaluated by measuring the myocardial infarct size as a percentage of the area at risk. The major findings were that regardless of sex, WT mice exhibited robust IPC with significantly smaller myocardial infarction, whereas eNOS -/- mice did not. IPC-induced cardiac protection was absent in eNOS-/- mice of both Jackson and Harvard origin. In general, female WT mice had smaller infarctions compared with male WT mice. Although prolonged ischemia caused significantly larger infarctions in WT mice of both sexes, they were consistently protected by IPC. Importantly, prolonged myocardial ischemia was associated with increased mortality in eNOS-/- mice, and the survival rate was higher in female eNOS-/- mice compared with male eNOS-/- mice. In conclusion, IPC protects WT mice against in vivo myocardial ischemia-reperfusion injury regardless of sex and ischemic duration, but the deletion of eNOS abolishes the cardioprotective effect of classical IPC.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume299
Issue number2
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Ischemia
Reperfusion
Infarction
Myocardial Ischemia
Myocardial Infarction
Myocardial Reperfusion Injury
Nitric Oxide Synthase Type III
Reperfusion Injury
Knockout Mice
Nitric Oxide
Survival Rate

Keywords

  • Cardioprotection
  • Endothelial nitric oxide synthase
  • Gender
  • Ischemic periods
  • Mortality

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

eNOS is required for acute in vivo ischemic preconditioning of the heart : Effects of ischemic duration and sex. / Talukder, M. A Hassan; Yang, Fuchun; Shimokawa, Hiroaki; Zweier, Jay L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 299, No. 2, 08.2010.

Research output: Contribution to journalArticle

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