Enlazin, a natural fusion of two classes of canonical cytoskeletal proteins, contributes to cytokinesis dynamics

Edelyn Octtaviani, Janet C. Effler, Douglas Robinson

Research output: Contribution to journalArticle

Abstract

Cytokinesis requires a complex network of equatorial and global proteins to regulate cell shape changes. Here, using interaction genetics, we report the first characterization of a novel protein, enlazin. Enlazin is a natural fusion of two canonical classes of actin-associated proteins, the ezrin-radixin-moesin family and fimbrin, and it is localized to actin-rich structures. A fragment of enlazin, enl-tr, was isolated as a genetic suppressor of the cytokinesis defect of cortexillin-I mutants. Expression of enl-tr disrupts expression of endogenous enlazin, indicating that enl-tr functions as a dominant-negative lesion. Enlazin is distributed globally during cytokinesis and is required for cortical tension and cell adhesion. Consistent with a role in cell mechanics, inhibition of enlazin in a cortexillin-I background restores cytokinesis furrowing dynamics and suppresses the growth-in-suspension defect. However, as expected for a role in cell adhesion, inhibiting enlazin in a myosin-II background induces a synthetic cytokinesis phenotype, frequently arresting furrow ingression at the dumbbell shape and/or causing recession of the furrow. Thus, enlazin has roles in cell mechanics and adhesion, and these roles seem to be differentially significant for cytokinesis, depending on the genetic background.

Original languageEnglish (US)
Pages (from-to)5275-5286
Number of pages12
JournalMolecular Biology of the Cell
Volume17
Issue number12
DOIs
StatePublished - Dec 2006

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Cytoskeletal Proteins
Cytokinesis
Cell Adhesion
Mechanics
Actins
Myosin Type II
Proteins
Cell Shape
Suspensions
Phenotype
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Enlazin, a natural fusion of two classes of canonical cytoskeletal proteins, contributes to cytokinesis dynamics. / Octtaviani, Edelyn; Effler, Janet C.; Robinson, Douglas.

In: Molecular Biology of the Cell, Vol. 17, No. 12, 12.2006, p. 5275-5286.

Research output: Contribution to journalArticle

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