Enkephalin: Radioimmunoassay and radioreceptor assay in morphine dependent rats

Steven R. Childers; Rabi Simantov; Solomon H. Snyder

doi:10.1016/0014-2999(77)90346-6

Enkephalin: Radioimmunoassay and radioreceptor assay in morphine dependent rats

Steven R. Childers, Rabi Simantov, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

A sensitive and specific radioimmunoassay for enkephalins is described which discriminates between leucine-enkephalin and methionine-enkephalin. Total opiod peptide activity was assayed by the ability of brain extracts to complete for opiate receptor binding. In rats treated with morphine and/or naloxone, opiates were separated from opioid peptides by Dowex AG-50W column chromatography prior to radioreceptor assay. Chronic administration of morphine failed to alter immunoreactive enkephalin levels or total opioid activity in radioreceptor assays.

Original language	English (US)
Pages (from-to)	289-293
Number of pages	5
Journal	European Journal of Pharmacology
Volume	46
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(77)90346-6
State	Published - Dec 1 1977

Keywords

Addiction
Enkephalin
Morphine
Opioid peptides

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(77)90346-6

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title = "Enkephalin: Radioimmunoassay and radioreceptor assay in morphine dependent rats",

abstract = "A sensitive and specific radioimmunoassay for enkephalins is described which discriminates between leucine-enkephalin and methionine-enkephalin. Total opiod peptide activity was assayed by the ability of brain extracts to complete for opiate receptor binding. In rats treated with morphine and/or naloxone, opiates were separated from opioid peptides by Dowex AG-50W column chromatography prior to radioreceptor assay. Chronic administration of morphine failed to alter immunoreactive enkephalin levels or total opioid activity in radioreceptor assays.",

keywords = "Addiction, Enkephalin, Morphine, Opioid peptides",

author = "Childers, {Steven R.} and Rabi Simantov and Snyder, {Solomon H.}",

note = "Funding Information: Supported by USPHS grants DA-01645 and DA-00266. We thank Adele M. Snowman for excellent technical assistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1977",

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day = "1",

doi = "10.1016/0014-2999(77)90346-6",

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TY - JOUR

T1 - Enkephalin

T2 - Radioimmunoassay and radioreceptor assay in morphine dependent rats

AU - Childers, Steven R.

AU - Simantov, Rabi

AU - Snyder, Solomon H.

PY - 1977/12/1

Y1 - 1977/12/1

N2 - A sensitive and specific radioimmunoassay for enkephalins is described which discriminates between leucine-enkephalin and methionine-enkephalin. Total opiod peptide activity was assayed by the ability of brain extracts to complete for opiate receptor binding. In rats treated with morphine and/or naloxone, opiates were separated from opioid peptides by Dowex AG-50W column chromatography prior to radioreceptor assay. Chronic administration of morphine failed to alter immunoreactive enkephalin levels or total opioid activity in radioreceptor assays.

AB - A sensitive and specific radioimmunoassay for enkephalins is described which discriminates between leucine-enkephalin and methionine-enkephalin. Total opiod peptide activity was assayed by the ability of brain extracts to complete for opiate receptor binding. In rats treated with morphine and/or naloxone, opiates were separated from opioid peptides by Dowex AG-50W column chromatography prior to radioreceptor assay. Chronic administration of morphine failed to alter immunoreactive enkephalin levels or total opioid activity in radioreceptor assays.

KW - Addiction

KW - Enkephalin

KW - Morphine

KW - Opioid peptides

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Enkephalin: Radioimmunoassay and radioreceptor assay in morphine dependent rats

Abstract

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