Enkephalin: Radioimmunoassay and radioreceptor assay in morphine dependent rats

Steven R. Childers, Rabi Simantov, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

A sensitive and specific radioimmunoassay for enkephalins is described which discriminates between leucine-enkephalin and methionine-enkephalin. Total opiod peptide activity was assayed by the ability of brain extracts to complete for opiate receptor binding. In rats treated with morphine and/or naloxone, opiates were separated from opioid peptides by Dowex AG-50W column chromatography prior to radioreceptor assay. Chronic administration of morphine failed to alter immunoreactive enkephalin levels or total opioid activity in radioreceptor assays.

Original languageEnglish (US)
Pages (from-to)289-293
Number of pages5
JournalEuropean Journal of Pharmacology
Volume46
Issue number3
DOIs
StatePublished - Dec 1 1977

Keywords

  • Addiction
  • Enkephalin
  • Morphine
  • Opioid peptides

ASJC Scopus subject areas

  • Pharmacology

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