Enkephalin convertase localization by [3H]guanidinoethylmercaptosuccinic acid autoradiography: Selective association with enkephalin-containing neurons

D. R. Lynch, S. M. Strittmatter, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Enkephalin convertase, an enkephalin-forming carboxypeptidase, is potently inhibited by guanidinoethylmercaptosuccinic acid (GEMSA). We have localized enkephalin convertase in rat brain by in vitro autoradiography with [3H]GEMSA. [3H]GEMSA-associated silver grains are highly concentrated in the median eminence, bed nucleus of the stria terminalis, lateral septum, dentate gyrus, hippocampus, central nucleus of the amygdala, preoptic hypothalamus, magnocellular nuclei of the hypothalamus, interpeduncular nucleus, dorsal parabrachial nucleus, locus coeruleus, nucleus of the solitary tract, and the substantia gelatinosa of the spinal trigeminal tract. This distribution corresponds closely with immunocytochemical localizations of enkephalin-containing cells and axons, indicating that enkephalin convertase is selectively involved in enkephalin biosynthesis.

Original languageEnglish (US)
Pages (from-to)6543-6547
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number20 I
StatePublished - 1984

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Carboxypeptidase H
Enkephalins
Autoradiography
Neurons
Hypothalamus
Substantia Gelatinosa
Septal Nuclei
Parahippocampal Gyrus
Median Eminence
Solitary Nucleus
Locus Coeruleus
Dentate Gyrus
Silver
Axons
guanidinoethylmercaptosuccinic acid
Brain

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Enkephalin convertase localization by [3H]guanidinoethylmercaptosuccinic acid autoradiography: Selective association with enkephalin-containing neurons",
abstract = "Enkephalin convertase, an enkephalin-forming carboxypeptidase, is potently inhibited by guanidinoethylmercaptosuccinic acid (GEMSA). We have localized enkephalin convertase in rat brain by in vitro autoradiography with [3H]GEMSA. [3H]GEMSA-associated silver grains are highly concentrated in the median eminence, bed nucleus of the stria terminalis, lateral septum, dentate gyrus, hippocampus, central nucleus of the amygdala, preoptic hypothalamus, magnocellular nuclei of the hypothalamus, interpeduncular nucleus, dorsal parabrachial nucleus, locus coeruleus, nucleus of the solitary tract, and the substantia gelatinosa of the spinal trigeminal tract. This distribution corresponds closely with immunocytochemical localizations of enkephalin-containing cells and axons, indicating that enkephalin convertase is selectively involved in enkephalin biosynthesis.",
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journal = "Proceedings of the National Academy of Sciences of the United States of America",
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T1 - Enkephalin convertase localization by [3H]guanidinoethylmercaptosuccinic acid autoradiography

T2 - Selective association with enkephalin-containing neurons

AU - Lynch, D. R.

AU - Strittmatter, S. M.

AU - Snyder, Solomon H

PY - 1984

Y1 - 1984

N2 - Enkephalin convertase, an enkephalin-forming carboxypeptidase, is potently inhibited by guanidinoethylmercaptosuccinic acid (GEMSA). We have localized enkephalin convertase in rat brain by in vitro autoradiography with [3H]GEMSA. [3H]GEMSA-associated silver grains are highly concentrated in the median eminence, bed nucleus of the stria terminalis, lateral septum, dentate gyrus, hippocampus, central nucleus of the amygdala, preoptic hypothalamus, magnocellular nuclei of the hypothalamus, interpeduncular nucleus, dorsal parabrachial nucleus, locus coeruleus, nucleus of the solitary tract, and the substantia gelatinosa of the spinal trigeminal tract. This distribution corresponds closely with immunocytochemical localizations of enkephalin-containing cells and axons, indicating that enkephalin convertase is selectively involved in enkephalin biosynthesis.

AB - Enkephalin convertase, an enkephalin-forming carboxypeptidase, is potently inhibited by guanidinoethylmercaptosuccinic acid (GEMSA). We have localized enkephalin convertase in rat brain by in vitro autoradiography with [3H]GEMSA. [3H]GEMSA-associated silver grains are highly concentrated in the median eminence, bed nucleus of the stria terminalis, lateral septum, dentate gyrus, hippocampus, central nucleus of the amygdala, preoptic hypothalamus, magnocellular nuclei of the hypothalamus, interpeduncular nucleus, dorsal parabrachial nucleus, locus coeruleus, nucleus of the solitary tract, and the substantia gelatinosa of the spinal trigeminal tract. This distribution corresponds closely with immunocytochemical localizations of enkephalin-containing cells and axons, indicating that enkephalin convertase is selectively involved in enkephalin biosynthesis.

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