Enkephalin convertase demonstrated in the pituitary and adrenal gland by [3H]guanidinoethylmercaptosuccinic acid autoradiography: Dehydration decreases neurohypophyseal levels

S. M. Strittmatter, D. R. Lynch, E. B. De Souza, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

[3H]Guanidinoethylmercaptosuccinic acid (GEMSA) autoradiography demonstrates the particulate form of a carboxypeptidase B-like peptide processing enzyme, enkephalin convertase (EC 3.4.17.10), in the rat pituitary and adrenal glands. The maximal number of binding sites (B(max)) for [3H]GEMSA is 20 pmol/mg protein in the intermediate lobe of the pituitary, 12.0 pmol/mg protein in the posterior pituitary lobe, 15 pmol/mg protein in the anterior pituitary lobe, 5.8 pmol/mg protein in the adrenal medulla and less than 0.3 pmol/mg protein in the adrenal cortex. The labeling pattern is homogeneous within each of these regions. Subcellular fractionation of the bovine adrenal medulla demonstrates that [3H]GEMSA-binding sites are localized to chromaffin granules. In Brattleboro rats and dehydrated rats, the level of posterior pituitary [3H]GEMSA binding is less than 25% of that in control animals. This decrease is abolished by arginine vasopressin treatment of Brattleboro rats or rehydration of dehydrated rats. There are no changes in [3H]GEMSA binding in the supraoptic nucleus or magnocellular portion of the paraventricular nucleus of the hypothalamus under any of these conditions, suggesting that the alterations observed in the neurohypophysis result from an increased rate of loss of enkephalin convertase. The level of anterior pituitary enkephalin convertase is unchanged by dehydration, adrenalectomy, or dexamethasone or in Brattleboro rats. [3H]GEMSA labeling in the intermediate pituitary lobe is unaffected by dehydration and haloperidol treatment and in Brattleboro rats. The adrenal medullary enzyme is not altered by reserpine, hypophysectomy, or splanchnic denervation or in Brattleboro rats.

Original languageEnglish (US)
Pages (from-to)1667-1674
Number of pages8
JournalEndocrinology
Volume117
Issue number4
StatePublished - 1985

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Carboxypeptidase H
Brattleboro Rats
Pituitary Gland
Adrenal Glands
Autoradiography
Dehydration
Intermediate Pituitary Gland
Posterior Pituitary Gland
Adrenal Medulla
Proteins
Binding Sites
Carboxypeptidase B
Chromaffin Granules
Supraoptic Nucleus
Hypophysectomy
Anterior Pituitary Gland
Viscera
Arginine Vasopressin
Paraventricular Hypothalamic Nucleus
Fluid Therapy

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Enkephalin convertase demonstrated in the pituitary and adrenal gland by [3H]guanidinoethylmercaptosuccinic acid autoradiography : Dehydration decreases neurohypophyseal levels. / Strittmatter, S. M.; Lynch, D. R.; De Souza, E. B.; Snyder, Solomon H.

In: Endocrinology, Vol. 117, No. 4, 1985, p. 1667-1674.

Research output: Contribution to journalArticle

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abstract = "[3H]Guanidinoethylmercaptosuccinic acid (GEMSA) autoradiography demonstrates the particulate form of a carboxypeptidase B-like peptide processing enzyme, enkephalin convertase (EC 3.4.17.10), in the rat pituitary and adrenal glands. The maximal number of binding sites (B(max)) for [3H]GEMSA is 20 pmol/mg protein in the intermediate lobe of the pituitary, 12.0 pmol/mg protein in the posterior pituitary lobe, 15 pmol/mg protein in the anterior pituitary lobe, 5.8 pmol/mg protein in the adrenal medulla and less than 0.3 pmol/mg protein in the adrenal cortex. The labeling pattern is homogeneous within each of these regions. Subcellular fractionation of the bovine adrenal medulla demonstrates that [3H]GEMSA-binding sites are localized to chromaffin granules. In Brattleboro rats and dehydrated rats, the level of posterior pituitary [3H]GEMSA binding is less than 25{\%} of that in control animals. This decrease is abolished by arginine vasopressin treatment of Brattleboro rats or rehydration of dehydrated rats. There are no changes in [3H]GEMSA binding in the supraoptic nucleus or magnocellular portion of the paraventricular nucleus of the hypothalamus under any of these conditions, suggesting that the alterations observed in the neurohypophysis result from an increased rate of loss of enkephalin convertase. The level of anterior pituitary enkephalin convertase is unchanged by dehydration, adrenalectomy, or dexamethasone or in Brattleboro rats. [3H]GEMSA labeling in the intermediate pituitary lobe is unaffected by dehydration and haloperidol treatment and in Brattleboro rats. The adrenal medullary enzyme is not altered by reserpine, hypophysectomy, or splanchnic denervation or in Brattleboro rats.",
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