TY - JOUR
T1 - Enkephalin convertase
T2 - characterization and localization using [3H]guanidinoethylmercaptosuccinic acid
AU - Lynch, David R.
AU - Venable, J. Clark
AU - Strittmatter, Stephen M.
AU - Snyder, Solomon H.
PY - 1988/1
Y1 - 1988/1
N2 - Enkephalin convertase (carboxypeptidase E, H; EC 3.4.17.10) is a carboxypeptidase B-like enzyme which appears to be physiologically associated with the biosynthesis of the enkephalins and certain other peptides. We have localized enkephalin convertase in the brain and other tissues autoradiographically by labeling studies with [3H]guanidinoethylmercaptosuccinic acid ([3H]GEMSA). In the brain, [3H]GEMSA localizations parallel enkephalin distribution but with certain exceptions, suggesting a role in relation to other peptides. In the pancreas, [3H]GEMSA binding sites are localized to the islets suggesting an involvement in insulin, glucagon, or somatostatin formation. The selective concentration of [3H]GEMSA grains in cardiac atria suggests a link to atrial natriuretic factor.
AB - Enkephalin convertase (carboxypeptidase E, H; EC 3.4.17.10) is a carboxypeptidase B-like enzyme which appears to be physiologically associated with the biosynthesis of the enkephalins and certain other peptides. We have localized enkephalin convertase in the brain and other tissues autoradiographically by labeling studies with [3H]guanidinoethylmercaptosuccinic acid ([3H]GEMSA). In the brain, [3H]GEMSA localizations parallel enkephalin distribution but with certain exceptions, suggesting a role in relation to other peptides. In the pancreas, [3H]GEMSA binding sites are localized to the islets suggesting an involvement in insulin, glucagon, or somatostatin formation. The selective concentration of [3H]GEMSA grains in cardiac atria suggests a link to atrial natriuretic factor.
KW - neuropeptide processing
KW - opiates
KW - spinal cord
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U2 - 10.1016/0300-9084(88)90158-7
DO - 10.1016/0300-9084(88)90158-7
M3 - Article
C2 - 3135843
AN - SCOPUS:0023936474
SN - 0300-9084
VL - 70
SP - 57
EP - 64
JO - Biochimie
JF - Biochimie
IS - 1
ER -