Enkephalin convertase: characterization and localization using [3H]guanidinoethylmercaptosuccinic acid

David R. Lynch, J. Clark Venable, Stephen M. Strittmatter, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Enkephalin convertase (carboxypeptidase E, H; EC 3.4.17.10) is a carboxypeptidase B-like enzyme which appears to be physiologically associated with the biosynthesis of the enkephalins and certain other peptides. We have localized enkephalin convertase in the brain and other tissues autoradiographically by labeling studies with [3H]guanidinoethylmercaptosuccinic acid ([3H]GEMSA). In the brain, [3H]GEMSA localizations parallel enkephalin distribution but with certain exceptions, suggesting a role in relation to other peptides. In the pancreas, [3H]GEMSA binding sites are localized to the islets suggesting an involvement in insulin, glucagon, or somatostatin formation. The selective concentration of [3H]GEMSA grains in cardiac atria suggests a link to atrial natriuretic factor.

Original languageEnglish (US)
Pages (from-to)57-64
Number of pages8
JournalBiochimie
Volume70
Issue number1
DOIs
StatePublished - 1988

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Keywords

  • neuropeptide processing
  • opiates
  • spinal cord

ASJC Scopus subject areas

  • Biochemistry

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