Enhancement of monocyte procoagulant activity by adhesion on vascular smooth muscle cells and intercellular adhesion molecule-1-transfected Chinese hamster ovary cells

Nikolaus Marx, Franz Josef Neumann, Dietlind Zohlnhöfer, Timm Dickfeld, Almuth Fischer, Susanne Heimerl, Albert Schömig

Research output: Contribution to journalArticle

Abstract

Background - Plaque erosion is a frequent finding in sudden death due to coronary thrombosis. The present study sought to investigate whether monocyte adhesion to human aortic vascular smooth muscle cells (VSMCs) induces procoagulant activity (PCA) and whether this could be mediated by intercellular adhesion molecule-1 (ICAM-1). Methods and Results - We incubated mononuclear cells (MNCs) with VSMCs and ICAM-1-transfected Chinese hamster ovary (CHO) cells, investigated monocyte tissue factor (TF) mRNA expression by Northern blot analysis and TF protein expression by ELISA, and measured PCA. Incubation of MNCs with VSMCs for 6 hours increased PCA from 0.7±0.1 to 166.0±37.9 mU/105 cells (P=0.007), which could be inhibited in a dose-dependent manner by the addition of blocking anti-ICAM-1 monoclonal antibodies. Prestimulation of VSMCs with interleukin-1β enhanced surface ICAM-1 expression significantly but did not induce PCA in VSMCs. Incubation of MNCs with prestimulated VSMCs led to a further increase in PCA to 239.9±27.9 mU/105 cells (P=0.02 compared with incubation with unstimulated VSMCs). Incubation of MNCs with VSMCs enhanced TF mRNA after 2 hours and significantly increased TF protein content after 6 hours. Incubation of purified monocytes with ICAM-1-transfected CHO cells increased PCA from 1.2±0.2 to 81.9±3.3 mU/105 cells (P2-integrin-mediated monocyte adhesion to ICAM-1 on VSMCs.

Original languageEnglish (US)
Pages (from-to)906-911
Number of pages6
JournalCirculation
Volume98
Issue number9
StatePublished - Sep 1 1998
Externally publishedYes

Keywords

  • Cell adhesion molecules
  • Cells
  • Coagulation
  • Muscle, smooth
  • Tissue

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Marx, N., Neumann, F. J., Zohlnhöfer, D., Dickfeld, T., Fischer, A., Heimerl, S., & Schömig, A. (1998). Enhancement of monocyte procoagulant activity by adhesion on vascular smooth muscle cells and intercellular adhesion molecule-1-transfected Chinese hamster ovary cells. Circulation, 98(9), 906-911.