Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: A novel approach for inducing recognition of new viral epitopes

Moiz Kitabwalla; Francois Villinger; Hassibullah Akeefe; Zhaohao Liao; Ann E. Mayne; Lisa Gargano; Adam P. Conner; Jo Ann Maltais; Gretchen Kunas; James E.K. Hildreth; Aftab A. Ansari; Marc Bellotti

doi:10.1016/j.vaccine.2005.04.038

Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: A novel approach for inducing recognition of new viral epitopes

Moiz Kitabwalla, Francois Villinger, Hassibullah Akeefe, Zhaohao Liao, Ann E. Mayne, Lisa Gargano, Adam P. Conner, Jo Ann Maltais, Gretchen Kunas, James E.K. Hildreth, Aftab A. Ansari, Marc Bellotti

School of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We tested the hypothesis that removal of viral lipids using diisopropylether can enhance antigenicity of SIVmac251. DIPE delipidation removed cholesterol from SIVmac251 without significant loss of viral protein or RNA. Mice immunized with the same SIV preparation but boosted with delipidated SIVmac251 exhibited significantly broader and higher cellular and humoral immune responses compared to live or AT-2-inactivated virus. As little as 1 μg (total protein) of delipidated virus was sufficient to induce such enhanced immune responses. Thus, solvent treated lentivirus may provide a novel strategy to generate immune responses to additional viral epitopes.

Original language	English (US)
Pages (from-to)	4666-4677
Number of pages	12
Journal	Vaccine
Volume	23
Issue number	38
DOIs	https://doi.org/10.1016/j.vaccine.2005.04.038
State	Published - Sep 7 2005

Keywords

Delipidation
SIV
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2005.04.038

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Kitabwalla, M., Villinger, F., Akeefe, H., Liao, Z., Mayne, A. E., Gargano, L., Conner, A. P., Maltais, J. A., Kunas, G., Hildreth, J. E. K., Ansari, A. A., & Bellotti, M. (2005). Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: A novel approach for inducing recognition of new viral epitopes. Vaccine, 23(38), 4666-4677. https://doi.org/10.1016/j.vaccine.2005.04.038

Kitabwalla, M, Villinger, F, Akeefe, H, Liao, Z, Mayne, AE, Gargano, L, Conner, AP, Maltais, JA, Kunas, G, Hildreth, JEK, Ansari, AA & Bellotti, M 2005, 'Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: A novel approach for inducing recognition of new viral epitopes', Vaccine, vol. 23, no. 38, pp. 4666-4677. https://doi.org/10.1016/j.vaccine.2005.04.038

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abstract = "We tested the hypothesis that removal of viral lipids using diisopropylether can enhance antigenicity of SIVmac251. DIPE delipidation removed cholesterol from SIVmac251 without significant loss of viral protein or RNA. Mice immunized with the same SIV preparation but boosted with delipidated SIVmac251 exhibited significantly broader and higher cellular and humoral immune responses compared to live or AT-2-inactivated virus. As little as 1 μg (total protein) of delipidated virus was sufficient to induce such enhanced immune responses. Thus, solvent treated lentivirus may provide a novel strategy to generate immune responses to additional viral epitopes.",

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author = "Moiz Kitabwalla and Francois Villinger and Hassibullah Akeefe and Zhaohao Liao and Mayne, {Ann E.} and Lisa Gargano and Conner, {Adam P.} and Maltais, {Jo Ann} and Gretchen Kunas and Hildreth, {James E.K.} and Ansari, {Aftab A.} and Marc Bellotti",

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doi = "10.1016/j.vaccine.2005.04.038",

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AU - Villinger, Francois

AU - Akeefe, Hassibullah

AU - Liao, Zhaohao

AU - Mayne, Ann E.

AU - Gargano, Lisa

AU - Conner, Adam P.

AU - Maltais, Jo Ann

AU - Kunas, Gretchen

AU - Hildreth, James E.K.

AU - Ansari, Aftab A.

AU - Bellotti, Marc

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AB - We tested the hypothesis that removal of viral lipids using diisopropylether can enhance antigenicity of SIVmac251. DIPE delipidation removed cholesterol from SIVmac251 without significant loss of viral protein or RNA. Mice immunized with the same SIV preparation but boosted with delipidated SIVmac251 exhibited significantly broader and higher cellular and humoral immune responses compared to live or AT-2-inactivated virus. As little as 1 μg (total protein) of delipidated virus was sufficient to induce such enhanced immune responses. Thus, solvent treated lentivirus may provide a novel strategy to generate immune responses to additional viral epitopes.

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KW - SIV

KW - Vaccine

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Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: A novel approach for inducing recognition of new viral epitopes

Abstract

Keywords

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