Enhancement of antigen-specific activation of CD8+ memory cytotoxic T cells by B cell-derived factors

F. J. Kos, A. Mullbacher

Research output: Contribution to journalArticle

Abstract

Purified CD8+ T cells from influenza A/WSN-immune BALB/c (H-2(d)) mice respond with the generation of secondary A/WSN-specific Tc cells in vitro when stimulated with a synthetic peptide (NPP) with a sequence derived from influenza A virus nucleoprotein with high affinity for K(d) class I MHC molecules. The process of the conversion of NPP-K(d)-responding Tc cell precursors into effector Tc cells in a population of CD8+ T cells occurs with no demonstrable requirements for accessory cells or their lymphokine products. The addition of culture supernatants from several mouse and human B cell lymphomas and LPS-activated normal mouse B cells to the culture of NPP-stimulated immune CD8+ T cells enhanced the induction of secondary Ag-specific Tc cells. None of the tested supernatants in the absence of Ag (NPP) induced cytolytic Tc cells, indicating that B cell-derived secretory factors can exert their activity only on Ag-exposed CD8+ T cells. The augmentatory effect of these supernatants on Ag-specific activation of memory CD8+ T cells was attributed to the synergism between B cell-derived factors and IL-2 which is produced endogenously in cultures of NPP-stimulated D8+ T cells. The possible role of B cell-derived helper factors is discussed.

Original languageEnglish (US)
Pages (from-to)410-420
Number of pages11
JournalImmunobiology
Volume186
Issue number5
StatePublished - 1992
Externally publishedYes

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B-Lymphocytes
T-Lymphocytes
Antigens
Lymphokines
B-Cell Lymphoma
Human Influenza
Interleukin-2
Cell Culture Techniques
Peptides
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Enhancement of antigen-specific activation of CD8+ memory cytotoxic T cells by B cell-derived factors. / Kos, F. J.; Mullbacher, A.

In: Immunobiology, Vol. 186, No. 5, 1992, p. 410-420.

Research output: Contribution to journalArticle

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