Enhancement by lipid A of mucosal immunogenicity of liposome-associated cholera toxin.

N. F. Pierce, J. B. Sacci, C. R. Alving, E. C. Richardson

Research output: Contribution to journalArticle

Abstract

Two methods that might enhance the mucosal immunogenicity of a protein antigen, cholera toxin (CT), were studied in rats: association of CT with liposomes, and coadministration of CT with lipid A. Enteric priming by CT was not enhanced when the antigen was trapped within liposomes or bound to their surface via GM1 ganglioside, nor was it improved when CT was mixed with lipid A or with liposomes containing lipid A. However, lipid A did enhance priming by liposome-associated CT when the lipid A was incorporated into CT-bearing liposomes. It is concluded that lipid A can act as an adjuvant for a local IgA response to a mucosally applied antigen, at least when lipid A and the antigen are associated on a liposome carrier.

Original languageEnglish (US)
Pages (from-to)563-566
Number of pages4
JournalReviews of Infectious Diseases
Volume6
Issue number4
StatePublished - Jul 1984
Externally publishedYes

Fingerprint

Lipid A
Cholera Toxin
Liposomes
Antigens
G(M1) Ganglioside
Immunoglobulin A

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Enhancement by lipid A of mucosal immunogenicity of liposome-associated cholera toxin. / Pierce, N. F.; Sacci, J. B.; Alving, C. R.; Richardson, E. C.

In: Reviews of Infectious Diseases, Vol. 6, No. 4, 07.1984, p. 563-566.

Research output: Contribution to journalArticle

Pierce, N. F. ; Sacci, J. B. ; Alving, C. R. ; Richardson, E. C. / Enhancement by lipid A of mucosal immunogenicity of liposome-associated cholera toxin. In: Reviews of Infectious Diseases. 1984 ; Vol. 6, No. 4. pp. 563-566.
@article{5bc2ead4cf8f4009b1f568bd7958624c,
title = "Enhancement by lipid A of mucosal immunogenicity of liposome-associated cholera toxin.",
abstract = "Two methods that might enhance the mucosal immunogenicity of a protein antigen, cholera toxin (CT), were studied in rats: association of CT with liposomes, and coadministration of CT with lipid A. Enteric priming by CT was not enhanced when the antigen was trapped within liposomes or bound to their surface via GM1 ganglioside, nor was it improved when CT was mixed with lipid A or with liposomes containing lipid A. However, lipid A did enhance priming by liposome-associated CT when the lipid A was incorporated into CT-bearing liposomes. It is concluded that lipid A can act as an adjuvant for a local IgA response to a mucosally applied antigen, at least when lipid A and the antigen are associated on a liposome carrier.",
author = "Pierce, {N. F.} and Sacci, {J. B.} and Alving, {C. R.} and Richardson, {E. C.}",
year = "1984",
month = "7",
language = "English (US)",
volume = "6",
pages = "563--566",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Enhancement by lipid A of mucosal immunogenicity of liposome-associated cholera toxin.

AU - Pierce, N. F.

AU - Sacci, J. B.

AU - Alving, C. R.

AU - Richardson, E. C.

PY - 1984/7

Y1 - 1984/7

N2 - Two methods that might enhance the mucosal immunogenicity of a protein antigen, cholera toxin (CT), were studied in rats: association of CT with liposomes, and coadministration of CT with lipid A. Enteric priming by CT was not enhanced when the antigen was trapped within liposomes or bound to their surface via GM1 ganglioside, nor was it improved when CT was mixed with lipid A or with liposomes containing lipid A. However, lipid A did enhance priming by liposome-associated CT when the lipid A was incorporated into CT-bearing liposomes. It is concluded that lipid A can act as an adjuvant for a local IgA response to a mucosally applied antigen, at least when lipid A and the antigen are associated on a liposome carrier.

AB - Two methods that might enhance the mucosal immunogenicity of a protein antigen, cholera toxin (CT), were studied in rats: association of CT with liposomes, and coadministration of CT with lipid A. Enteric priming by CT was not enhanced when the antigen was trapped within liposomes or bound to their surface via GM1 ganglioside, nor was it improved when CT was mixed with lipid A or with liposomes containing lipid A. However, lipid A did enhance priming by liposome-associated CT when the lipid A was incorporated into CT-bearing liposomes. It is concluded that lipid A can act as an adjuvant for a local IgA response to a mucosally applied antigen, at least when lipid A and the antigen are associated on a liposome carrier.

UR - http://www.scopus.com/inward/record.url?scp=0021453851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021453851&partnerID=8YFLogxK

M3 - Article

C2 - 6474016

AN - SCOPUS:0021453851

VL - 6

SP - 563

EP - 566

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 4

ER -