Enhanced uptake of [11C]TPMP in canine brain tumor: A PET study

Igal Madar, James H. Anderson, Zsolt Szabo, Ursula Scheffel, Pan Fu Kao, Hayden T. Ravert, Robert F. Dannals

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro studies have demonstrated the membrane potential-dependent enhanced uptake of phosphonium salts, including [3H]triphenylmethylphosphonium (TPMP), into mitochondria of carcinoma and glioma-derived tumor cells, suggesting the potential use of phosphonium salts as tracers for tumor imaging. This study characterizes the in vivo uptake of [11C]TPMP in canine brain glioma using PET. Methods: Dynamic paired PET studies of [11C]TPMP followed by [68Ga]ethylenediaminetetraacetic acid (EDTA) were performed 4 d before and 9 d after tumor cell inoculation. Graphical analysis was used to evaluate [11C]TPMP retention in tumor tissue. Distribution of tracer uptake was compared with tumor histological sections. Results: [11C]TPMP exhibited enhanced uptake and prolonged retention in tumor cells. Patlak plot was linear over the 20- to 95-min postinjection period (r = 0.97 ± 0.1). [68Ga]EDTA exhibited a gradual washout from the tumor tissue. The tumor-to-normal brain uptake ratio at 55 to 95 min postinjection was 47.5 for [11C]TPMP and 8.1 for [68Ga]EDTA. Qualitative comparison with histological sections indicated that [11C]TPMP enhanced uptake was restricted to the tumor area. Conclusion: The enhanced uptake and prolonged retention in tumor suggest [11C]TPMP as a promising means for imaging of gliomas in dogs. The need for studies in humans is indicated.

Original languageEnglish (US)
Pages (from-to)1180-1185
Number of pages6
JournalJournal of Nuclear Medicine
Volume40
Issue number7
StatePublished - Jul 1 1999

Keywords

  • Dog
  • Glioma
  • PET
  • [C]triphenylmethylphosphonium

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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