Enhanced resolution of experimental ards through il-4-mediated lung macrophage reprogramming

F. R. D’Alessio, J. M. Craig, B. D. Singer, D. C. Files, J. R. Mock, B. T. Garibaldi, J. Fallica, A. Tripathi, P. Mandke, J. H. Gans, N. Limjunyawong, V. K. Sidhaye, N. M. Heller, W. Mitzner, L. S. King, N. R. Aggarwal

Research output: Research - peer-reviewArticle

Abstract

Despite intense investigation, acute respiratory distress syndrome (ARDS) remains an enormous clinical problem for which no specific therapies currently exist. In this study, we used intratracheal lipopolysaccharide or Pseudomonas bacteria administration to model experimental acute lung injury (ALI) and to further understand mediators of the resolution phase of ARDS. Recent work demonstrates macrophages transition from a predominant proinflammatory M1 phenotype during acute inflammation to an anti-inflammatory M2 phenotype with ALI resolution. We tested the hypothesis that IL-4, a potent inducer of M2-specific protein expression, would accelerate ALI resolution and lung repair through reprogramming of endogenous inflammatory macrophages. In fact, IL-4 treatment was found to offer dramatic benefits following delayed administration to mice subjected to experimental ALI, including increased survival, accelerated resolution of lung injury, and improved lung function. Expression of the M2 proteins Arg1, FIZZ1, and Ym1 was increased in lung tissues following IL-4 treatment, and among macrophages, FIZZ1 was most prominently upregulated in the interstitial subpopulation. A similar trend was observed for the expression of macrophage mannose receptor (MMR) and Dectin-1 on the surface of alveolar macrophages following IL-4 administration. Macrophage depletion or STAT6 deficiency abrogated the therapeutic effect of IL-4. Collectively, these data demonstrate that IL-4-mediated therapeutic macrophage reprogramming can accelerate resolution and lung repair despite delayed use following experimental ALI. IL-4 or other therapies that target late-phase, proresolution pathways may hold promise for the treatment of human ARDS.

LanguageEnglish (US)
PagesL733-L746
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume310
Issue number8
DOIs
StatePublished - Apr 15 2016

Fingerprint

Interleukin-4
Macrophages
Lung
Acute Lung Injury
Adult Respiratory Distress Syndrome
Therapeutics
Phenotype
Proteins
Alveolar Macrophages
Lung Injury
Therapeutic Uses
Pseudomonas
Lipopolysaccharides
Anti-Inflammatory Agents
Theoretical Models
Inflammation
Bacteria
Survival
dectin 1
mannose receptor

Keywords

  • Acute lung injury
  • ARDS
  • Interleukin-4
  • Macrophage
  • Resolution

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)
  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology (medical)

Cite this

Enhanced resolution of experimental ards through il-4-mediated lung macrophage reprogramming. / D’Alessio, F. R.; Craig, J. M.; Singer, B. D.; Files, D. C.; Mock, J. R.; Garibaldi, B. T.; Fallica, J.; Tripathi, A.; Mandke, P.; Gans, J. H.; Limjunyawong, N.; Sidhaye, V. K.; Heller, N. M.; Mitzner, W.; King, L. S.; Aggarwal, N. R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 310, No. 8, 15.04.2016, p. L733-L746.

Research output: Research - peer-reviewArticle

D’Alessio, F. R. ; Craig, J. M. ; Singer, B. D. ; Files, D. C. ; Mock, J. R. ; Garibaldi, B. T. ; Fallica, J. ; Tripathi, A. ; Mandke, P. ; Gans, J. H. ; Limjunyawong, N. ; Sidhaye, V. K. ; Heller, N. M. ; Mitzner, W. ; King, L. S. ; Aggarwal, N. R./ Enhanced resolution of experimental ards through il-4-mediated lung macrophage reprogramming. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2016 ; Vol. 310, No. 8. pp. L733-L746
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