Enhanced Krev-1 expression inhibits the growth of pancreatic adenocarcinoma cells

Steven D. Leach, David H. Berger, B. Scott Davidson, Steven A. Curley, Michael A. Tainsky

Research output: Contribution to journalArticle

Abstract

Pancreatic ductal adenocarcinoma is characterized by a high rate of activating mutations involving codon 12 of the K-ras protooncogene. As a means of ras-targeted intervention, the effects of enhanced Krev-1 gene expression on the growth and tumorigenicity of the hamster pancreatic adenocarcinoma cell line PC-1 were evaluated. Overexpression of the Krev-1 gene product resulted in morphologic reversion to a less transformed phenotype, as well as retarded growth kinetics and diminished potential for anchorage-independent growth. Among six transfected cell lines, the magnitude of these changes correlated with the degree of Krev-1 overexpression as assessed by Western blot. When PC-1 cells overexpressing high levels of the Krev-1 gene product were assessed for tumorigenicity in syngeneic animals, an increased latency to tumor growth and a decreased tumor size were noted. The results confirm that overexpression of the Krev-1 gene may provide a useful strategy for ras-targeted intervention in this disease.

Original languageEnglish (US)
Pages (from-to)491-498
Number of pages8
JournalPancreas
Volume16
Issue number4
Publication statusPublished - May 1998
Externally publishedYes

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Keywords

  • Adenocarcinoma
  • Hamster
  • K-ras
  • Krev-1

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Leach, S. D., Berger, D. H., Davidson, B. S., Curley, S. A., & Tainsky, M. A. (1998). Enhanced Krev-1 expression inhibits the growth of pancreatic adenocarcinoma cells. Pancreas, 16(4), 491-498.