Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid

D. S. McLeod, D. J. Lefer, C. Merges, Gerard Anthony Lutty

Research output: Contribution to journalArticle

Abstract

Elevated expression of intercellular adhesion molecule-1 (ICAM-1) as well as E- and P-selectin occurs on the vascular endothelium in a number of disease states and its thought to play an early critical role in the adhesion of circulating leukocytes to the endothelium. The goal of the present study was to investigate the immunolocalization of these molecules in the retina and choroid of postmortem human tissue sections from nondiabetic and diabetic subjects. Whereas ICAM-1 was localized primarily within the choriocapillaris of nondiabetic subjects, immunoreactivity in diabetic was significantly elevated throughout the choroidal vasculature and within retinal blood vessels (P <0.05). In the choroid, P-selectin was most prominent in veins of the nondiabetic, whereas in diabetics, P-selectin was significantly elevated in arteries (P <0.001) and veins (P <0.05) and, in some cases, was also observed in choriocapillaris. P-selectin immunoreactivity was not observed in the retina of any subject. E-selectin immunoreactivity was not observed in choroid or retina in any subjects. Neutrophil numbers per square millimeter of tissue were significantly elevated in diabetic choroid (P <0.05) and retina (P <0.001). Our results demonstrate that ICAM-1 and P-selectin are constitutively expressed in the normal choroid and are upregulated in the choroidal vasculature in diabetes, but only ICAM-1 was upregulated in the retina of diabetic subjects. Increased cell adhesion molecule expression may contribute to the retinal and choroidal microangiopathy observed in diabetics by enhancing leukocyte adhesion and consequently the incidence of capillary obstruction and endothelial cell injury.

Original languageEnglish (US)
Pages (from-to)642-653
Number of pages12
JournalAmerican Journal of Pathology
Volume147
Issue number3
StatePublished - 1995

Fingerprint

P-Selectin
Choroid
Retina
Intercellular Adhesion Molecule-1
E-Selectin
Veins
Leukocytes
Retinal Vessels
Vascular Endothelium
Cell Adhesion Molecules
Endothelium
Neutrophils
Endothelial Cells
Arteries
Incidence
Wounds and Injuries

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid. / McLeod, D. S.; Lefer, D. J.; Merges, C.; Lutty, Gerard Anthony.

In: American Journal of Pathology, Vol. 147, No. 3, 1995, p. 642-653.

Research output: Contribution to journalArticle

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