Enhanced effect of combining human cardiac stem cells and bone marrow mesenchymal stem cells to reduce infarct size and to restore cardiac function after myocardial infarction

Adam R. Williams, Konstantinos E. Hatzistergos, Benjamin Addicott, Fred McCall, Decio Carvalho, Viky Suncion, Azorides R. Morales, Jose Da Silva, Mark A. Sussman, Alan W. Heldman, Joshua M. Hare

Research output: Contribution to journalArticle

Abstract

BACKGROUND - : Because mesenchymal stem cells (MSCs) induce proliferation and differentiation of c-kit cardiac stem cells (CSCs) in vivo and in vitro, we hypothesized that combining human (h) MSCs with c-kit hCSCs produces greater infarct size reduction compared with either cell administered alone after myocardial infarction (MI). METHODS AND RESULTS - : Yorkshire swine underwent balloon occlusion of the left anterior descending coronary artery followed by reperfusion and were immunosuppressed after MI with cyclosporine and methylprednisolone. Intramyocardial combination hCSCs/hMSCs (1 million cells/200 million cells, n=5), hCSCs alone (1 million cells, n=5), hMSCs alone (200 million cells, n=5), or placebo (phosphate-buffered saline; n=5) was injected into the infarct border zones at 14 days after MI. Phenotypic response to cell therapy was assessed by cardiac magnetic resonance imaging and micromanometer conductance catheterization hemodynamics. Although each cell therapy group had reduced MI size relative to placebo (P

Original languageEnglish (US)
Pages (from-to)213-223
Number of pages11
JournalCirculation
Volume127
Issue number2
DOIs
StatePublished - Jan 15 2013
Externally publishedYes

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Keywords

  • heart failure
  • mesenchymal stem cells
  • myocardial infarction
  • stem cells

ASJC Scopus subject areas

  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Williams, A. R., Hatzistergos, K. E., Addicott, B., McCall, F., Carvalho, D., Suncion, V., Morales, A. R., Da Silva, J., Sussman, M. A., Heldman, A. W., & Hare, J. M. (2013). Enhanced effect of combining human cardiac stem cells and bone marrow mesenchymal stem cells to reduce infarct size and to restore cardiac function after myocardial infarction. Circulation, 127(2), 213-223. https://doi.org/10.1161/CIRCULATIONAHA.112.131110