Enhanced differentiation of human neural crest stem cells towards the Schwann cell lineage by aligned electrospun fiber matrix

Yong Juan Ren, Shuming Zhang, Ruifa Mi, Qiuyue Liu, Xianmin Zeng, Mahendra Rao, Ahmet Hoke, Hai Quan Mao

Research output: Contribution to journalArticle

Abstract

Human pluripotent stem cell-derived neural crest stem cells (NCSCs) provide a promising cell source for generating Schwann cells in the treatment of neurodegenerative diseases and traumatic injuries in the peripheral nervous system. Influencing cell behavior through a synthetic matrix topography has been shown to be an effective approach to directing stem cell proliferation and differentiation. Here we have investigated the effect of nanofiber topography on the differentiation of human embryonic stem cellderived NCSCs towards the Schwann cell lineage. Using electrospun fibers of different diameters and alignments we demonstrated that aligned fiber matrices effectively induced cell alignment, and that fiber matrices with average diameters of 600 nm and 1.6 lm most effectively promoted NCSC differentiation towards the Schwann cell lineage compared with random fibers and two-dimensional tissue culture plates. More importantly, human NCSCs that were predifferentiated in Schwann cell medium for 2 weeks exhibited higher sensitivity to the aligned fiber topography than undifferentiated NCSCs. This study provides an efficient protocol for Schwann cell derivation by combining an aligned nanofiber matrix and an optimized differentiation medium, and highlights the importance of matching extrinsic matrix signaling with cell intrinsic programming in a temporally specific manner.

Original languageEnglish (US)
Pages (from-to)7727-7736
Number of pages10
JournalActa Biomaterialia
Volume9
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Neural Stem Cells
Neural Crest
Schwann Cells
Cell Lineage
Stem cells
Cells
Fibers
Nanofibers
Topography
Cell Differentiation
Pluripotent Stem Cells
Peripheral Nervous System
Neurodegenerative diseases
Neurodegenerative Diseases
Tissue culture
Stem Cells
Cell proliferation
Neurology
Cell Proliferation
Wounds and Injuries

Keywords

  • Alignment
  • Differentiation
  • Electrospun fibers
  • Neural crest stem cells
  • Schwann cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Enhanced differentiation of human neural crest stem cells towards the Schwann cell lineage by aligned electrospun fiber matrix. / Ren, Yong Juan; Zhang, Shuming; Mi, Ruifa; Liu, Qiuyue; Zeng, Xianmin; Rao, Mahendra; Hoke, Ahmet; Mao, Hai Quan.

In: Acta Biomaterialia, Vol. 9, No. 8, 08.2013, p. 7727-7736.

Research output: Contribution to journalArticle

Ren, Yong Juan ; Zhang, Shuming ; Mi, Ruifa ; Liu, Qiuyue ; Zeng, Xianmin ; Rao, Mahendra ; Hoke, Ahmet ; Mao, Hai Quan. / Enhanced differentiation of human neural crest stem cells towards the Schwann cell lineage by aligned electrospun fiber matrix. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 8. pp. 7727-7736.
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