Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection

Peter S. Backlund; Amina S. Woods; Jinsheng Dong; Alan G. Hennebusch; Alfred L. Yergey

Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection

Peter S. Backlund, Amina S. Woods, Jinsheng Dong, Alan G. Hennebusch, Alfred L. Yergey

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Peptide ionization was analyzed using MALDI mass spectrometry (MS) in the presence of ammonium salts to improve detection of phosphopeptides in peptide mixtures. Standard phosphopeptides from beta-casein and candidate novel phosphopeptides from the yeast protein Gcn2p were identified from MALDI MS by analysis of the relative intensities of positive and negative ions from the peptides. Beta casein were digested with trypsin to generate a peptide mixture containing known phosphopeptides. Ammonium sulfate increased the relative intensity of both phosphopeptides, while ammonium citrate invreased the intensity of phosphopeptide 16-40, but had no effect on phosphopeptide 48-63.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	289-290
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Backlund, PS, Woods, AS, Dong, J, Hennebusch, AG & Yergey, AL 2002, Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 289-290, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

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title = "Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection",

abstract = "Peptide ionization was analyzed using MALDI mass spectrometry (MS) in the presence of ammonium salts to improve detection of phosphopeptides in peptide mixtures. Standard phosphopeptides from beta-casein and candidate novel phosphopeptides from the yeast protein Gcn2p were identified from MALDI MS by analysis of the relative intensities of positive and negative ions from the peptides. Beta casein were digested with trypsin to generate a peptide mixture containing known phosphopeptides. Ammonium sulfate increased the relative intensity of both phosphopeptides, while ammonium citrate invreased the intensity of phosphopeptide 16-40, but had no effect on phosphopeptide 48-63.",

author = "Backlund, {Peter S.} and Woods, {Amina S.} and Jinsheng Dong and Hennebusch, {Alan G.} and Yergey, {Alfred L.}",

year = "2002",

language = "English (US)",

pages = "289--290",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection

AU - Backlund, Peter S.

AU - Woods, Amina S.

AU - Dong, Jinsheng

AU - Hennebusch, Alan G.

AU - Yergey, Alfred L.

PY - 2002

Y1 - 2002

N2 - Peptide ionization was analyzed using MALDI mass spectrometry (MS) in the presence of ammonium salts to improve detection of phosphopeptides in peptide mixtures. Standard phosphopeptides from beta-casein and candidate novel phosphopeptides from the yeast protein Gcn2p were identified from MALDI MS by analysis of the relative intensities of positive and negative ions from the peptides. Beta casein were digested with trypsin to generate a peptide mixture containing known phosphopeptides. Ammonium sulfate increased the relative intensity of both phosphopeptides, while ammonium citrate invreased the intensity of phosphopeptide 16-40, but had no effect on phosphopeptide 48-63.

AB - Peptide ionization was analyzed using MALDI mass spectrometry (MS) in the presence of ammonium salts to improve detection of phosphopeptides in peptide mixtures. Standard phosphopeptides from beta-casein and candidate novel phosphopeptides from the yeast protein Gcn2p were identified from MALDI MS by analysis of the relative intensities of positive and negative ions from the peptides. Beta casein were digested with trypsin to generate a peptide mixture containing known phosphopeptides. Ammonium sulfate increased the relative intensity of both phosphopeptides, while ammonium citrate invreased the intensity of phosphopeptide 16-40, but had no effect on phosphopeptide 48-63.

UR - http://www.scopus.com/inward/record.url?scp=2442655661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442655661&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442655661

SP - 289

EP - 290

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Enhanced detection of phosphopeptides by MALDI mass spectrometry by use of ammonium sulfate and negative ion detection

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