Abstract
Highly active antiretroviral therapy can decrease plasma HIV-1 levels to below the limit of detection. However, HIV-1 persists in latently infected resting-memory CD4+ T-cells carrying an integrated copy of the viral genome. The pool of latently infected cells is extremely stable and represents a major barrier to HIV-1 eradication. Identification and characterization of this reservoir required the development of methods for purifying resting CD4+ T-cells from HIV-1-infected individuals, activating the cells to induce virus production, and detecting and quantitating cells capable of releasing infectious virus. The development of an enhanced viral culture assay to quantitate the number of latently infected cells carrying replication competent virus is described here.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3-15 |
| Number of pages | 13 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 304 |
| State | Published - 2005 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Enhanced culture assay for detection and quantitation of latently infected, resting CD4+ T-cells carrying replication-competent virus in HIV-1-infected individuals. / Siliciano, Janet M; Siliciano, Robert F.
In: Methods in molecular biology (Clifton, N.J.), Vol. 304, 2005, p. 3-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced culture assay for detection and quantitation of latently infected, resting CD4+ T-cells carrying replication-competent virus in HIV-1-infected individuals.
AU - Siliciano, Janet M
AU - Siliciano, Robert F
PY - 2005
Y1 - 2005
N2 - Highly active antiretroviral therapy can decrease plasma HIV-1 levels to below the limit of detection. However, HIV-1 persists in latently infected resting-memory CD4+ T-cells carrying an integrated copy of the viral genome. The pool of latently infected cells is extremely stable and represents a major barrier to HIV-1 eradication. Identification and characterization of this reservoir required the development of methods for purifying resting CD4+ T-cells from HIV-1-infected individuals, activating the cells to induce virus production, and detecting and quantitating cells capable of releasing infectious virus. The development of an enhanced viral culture assay to quantitate the number of latently infected cells carrying replication competent virus is described here.
AB - Highly active antiretroviral therapy can decrease plasma HIV-1 levels to below the limit of detection. However, HIV-1 persists in latently infected resting-memory CD4+ T-cells carrying an integrated copy of the viral genome. The pool of latently infected cells is extremely stable and represents a major barrier to HIV-1 eradication. Identification and characterization of this reservoir required the development of methods for purifying resting CD4+ T-cells from HIV-1-infected individuals, activating the cells to induce virus production, and detecting and quantitating cells capable of releasing infectious virus. The development of an enhanced viral culture assay to quantitate the number of latently infected cells carrying replication competent virus is described here.
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UR - http://www.scopus.com/inward/citedby.url?scp=24944518559&partnerID=8YFLogxK
M3 - Article
C2 - 16061962
AN - SCOPUS:24944518559
VL - 304
SP - 3
EP - 15
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -