Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes

Jong J. Kirn, Mark L. Bagarazzi, Neil Trivedi, Yin Hu, Ken Kazahaya, Darren M. Wilson, Richard Ciccarelli, Michael A. Chattergoon, Kesan Dang, Sundarasamy Mahalingam, Ara A. Chalian, Michael G. Agadjanyan, Jean D. Boyer, Bin Wang, David B. Weiner

Research output: Contribution to journalArticlepeer-review

Abstract

Nucleic acid immunization is a novel vaccination technique to induce antigen-specific immune responses. We have developed expression cassettes for cell surface markers CD80 and CD86, two functionally related costimulatory molecules that play an important role in the induction of T cell-mediated immune responses. Coimmunization of these expression plasmids, along with plasmid DNA encoding for HIV-1 antigens, did not result in any significant change in the humoral response; however, we observed a dramatic increase in cytotoxic T-lymphocyte (CTL) induction as well as T-helper cell proliferation after the coadministration of CD86 genes, in contrast, coimmunization with a CD80 expression cassette resulted in a minor, but positive increase in T-helper cell or CTL responses. This strategy may be of value for the generation of rationally designed vaccines and immune therapeutics.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalNature biotechnology
Volume15
Issue number7
DOIs
StatePublished - Jul 1997
Externally publishedYes

Keywords

  • Costimulatory molecules
  • DNA vaccines
  • Immune responses

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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