Engineering of a superhelicase through conformational control

Sinan Arslan, Rustem Khafizov, Christopher D. Thomas, Yann R. Chemla, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Conformational control of biomolecular activities can reveal functional insights and enable the engineering of novel activities. Here we show that conformational control through intramolecular cross-linking of a helicase monomer with undetectable unwinding activity converts it into a superhelicase that can unwind thousands of base pairs processively, even against a large opposing force. A natural partner that enhances the helicase activity is shown to achieve its stimulating role also by selectively stabilizing the active conformation. Our work provides insight into the regulation of nucleic acid unwinding activity and introduces a monomeric superhelicase without nuclease activities, which may be useful for biotechnological applications.

Original languageEnglish (US)
Pages (from-to)344-347
Number of pages4
JournalScience
Volume348
Issue number6232
DOIs
StatePublished - Apr 17 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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