Engineering bone tissue from human embryonic stem cells

Darja Marolt, Iván Marcos Campos, Sarindr Bhumiratana, Ana Koren, Petros Petridis, Geping Zhang, Patrice F. Spitalnik, Warren L Grayson, Gordana Vunjak-Novakovic

Research output: Contribution to journalArticle

Abstract

In extensive bone defects, tissue damage and hypoxia lead to cell death, resulting in slow and incomplete healing. Human embryonic stem cells (hESC) can give rise to all specialized lineages found in healthy bone and are therefore uniquely suited to aid regeneration of damaged bone. We show that the cultivation of hESC-derived mesenchymal progenitors on 3D osteoconductive scaffolds in bioreactors with medium perfusion leads to the formation of large and compact bone constructs. Notably, the implantation of engineered bone in immunodeficient mice for 8 wk resulted in the maintenance and maturation of bone matrix, without the formation of teratomas that is consistently observed when undifferentiated hESCs are implanted, alone or in bone scaffolds. Our study provides a proof of principle that tissue-engineering protocols can be successfully applied to hESC progenitors to grow bone grafts for use in basic and translational studies.

Original languageEnglish (US)
Pages (from-to)8705-8709
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number22
DOIs
Publication statusPublished - May 29 2012

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Keywords

  • Pluripotent stem cells
  • Tissue regeneration

ASJC Scopus subject areas

  • General

Cite this

Marolt, D., Campos, I. M., Bhumiratana, S., Koren, A., Petridis, P., Zhang, G., ... Vunjak-Novakovic, G. (2012). Engineering bone tissue from human embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8705-8709. https://doi.org/10.1073/pnas.1201830109