Engineered Models of Confined Cell Migration

Colin D. Paul, Wei Chien Hung, Denis Wirtz, Konstantinos Konstantopoulos

Research output: Contribution to journalReview articlepeer-review

Abstract

Cells in the body are physically confined by neighboring cells, tissues, and the extracellular matrix. Although physical confinement modulates intracellular signaling and the underlying mechanisms of cell migration, it is difficult to study in vivo. Furthermore, traditional two-dimensional cell migration assays do not recapitulate the complex topographies found in the body. Therefore, a number of experimental in vitro models that confine and impose forces on cells in well-defined microenvironments have been engineered. We describe the design and use of microfluidic microchannel devices, grooved substrates, micropatterned lines, vertical confinement devices, patterned hydrogels, and micropipette aspiration assays for studying cell responses to confinement. Use of these devices has enabled the delineation of changes in cytoskeletal reorganization, cell-substrate adhesions, intracellular signaling, nuclear shape, and gene expression that result from physical confinement. These assays and the physiologically relevant signaling pathways that have been elucidated are beginning to have a translational and clinical impact.

Original languageEnglish (US)
Pages (from-to)159-180
Number of pages22
JournalAnnual Review of Biomedical Engineering
Volume18
DOIs
StatePublished - Jul 11 2016

Keywords

  • Cell confinement
  • Cell migration
  • Cytoskeletal organization
  • Mechanosensing
  • Microfluidics
  • Micropatterning

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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