Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells

Jonghoon Choi, Hoyoung Park, Taeho Kim, Yoon Jeong, Myoung H.wan Oh, Taeghwan Hyeon, Assaf A. Gilad, Kwan H.yi Lee

Research output: Contribution to journalArticle

Abstract

We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen hydrogels were fabricated under a variety of conditions in order to optimize the release profile of biomolecules and nanoparticles for the desired duration and amount. The collagen constructs containing biomolecules/nanoconstructs were incubated under physiological conditions (ie, 37°C and 5% CO2) for 24 hours, and the release profile was tuned from 20% to 70% of initially loaded materials by varying the gelation conditions of the collagen constructs. The amounts of released biomolecules and nanoparticles were quantified respectively by measuring the intensity of fluorescence and X-ray scattering. The collagen hydrogel we fabricated may serve as an efficient platform for the controlled release of biomolecules and imaging agents in human gingiva to facilitate the regeneration of oral tissues.

Original languageEnglish (US)
Pages (from-to)5189-5201
Number of pages13
JournalInternational journal of nanomedicine
Volume9
DOIs
StatePublished - Jan 1 2014

Keywords

  • biomolecules
  • engineered collagen hydrogels
  • growth
  • human gingival cells
  • imaging agents
  • sustained release

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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