Energy state and vasomotor tone in hypoxic pig lungs

Philip Buescher, David B Pearse, R. P. Pillai, M. C. Litt, M. C. Mitchell, J. T. Sylvester

Research output: Contribution to journalArticle

Abstract

To evaluate the role of energy state in pulmonary vascular responses to hypoxia, we exposed isolated pig lungs to decreases in inspired P(O2) or increases in perfusate NaCN concentration. Lung energy state was assessed by 31P nuclear magnetic resonance spectroscopy or measurement of adenine nucleotides by high-pressure liquid chromatography in freeze-clamped biopsies. In ventilated lungs, inspired P(O2) of 200 (normoxia), 50 (hypoxia), and 0 Torr (anoxia) did not change adenine nucleotides but resulted in steady-state pulmonary arterial pressure (Ppa) values of 15.5 ± 1.4, 30.3 ± 1.8, and 17.2 ± 1.9 mmHg, respectively, indicating vasoconstriction during hypoxia and reversal of vasoconstriction during anoxia. In degassed lungs, similar changes in Ppa were observed; however, energy state deteriorated during anoxia. An increase in perfusate NaCN concentration from 0 to 0.1 mM progressively increased Ppa and did not alter adenine nucleotides, whereas 1 mM reversed this vasoconstriction and caused deterioration of energy state. These results suggest that 1) pulmonary vasoconstrictor responses to hypoxia or cyanide occurred independently of whole lung energy state, 2) the inability of the pulmonary vasculature to sustain hypoxic vasoconstriction during anoxia might be associated with decreased energy state in some lung compartment, and 3) atelectasis was detrimental to whole lung energy state.

Original languageEnglish (US)
Pages (from-to)1874-1881
Number of pages8
JournalJournal of Applied Physiology
Volume70
Issue number4
StatePublished - 1991

Fingerprint

Swine
Lung
Vasoconstriction
Adenine Nucleotides
Pulmonary Atelectasis
Hypoxia
Cyanides
Vasoconstrictor Agents
Blood Vessels
Arterial Pressure
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Biopsy

Keywords

  • adenosine 5'-triphosphate
  • anoxia
  • cyanide
  • hypoxia
  • phosphorus-31 nuclear magnetic resonance spectroscopy
  • pulmonary arterial pressure

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Buescher, P., Pearse, D. B., Pillai, R. P., Litt, M. C., Mitchell, M. C., & Sylvester, J. T. (1991). Energy state and vasomotor tone in hypoxic pig lungs. Journal of Applied Physiology, 70(4), 1874-1881.

Energy state and vasomotor tone in hypoxic pig lungs. / Buescher, Philip; Pearse, David B; Pillai, R. P.; Litt, M. C.; Mitchell, M. C.; Sylvester, J. T.

In: Journal of Applied Physiology, Vol. 70, No. 4, 1991, p. 1874-1881.

Research output: Contribution to journalArticle

Buescher, P, Pearse, DB, Pillai, RP, Litt, MC, Mitchell, MC & Sylvester, JT 1991, 'Energy state and vasomotor tone in hypoxic pig lungs', Journal of Applied Physiology, vol. 70, no. 4, pp. 1874-1881.
Buescher P, Pearse DB, Pillai RP, Litt MC, Mitchell MC, Sylvester JT. Energy state and vasomotor tone in hypoxic pig lungs. Journal of Applied Physiology. 1991;70(4):1874-1881.
Buescher, Philip ; Pearse, David B ; Pillai, R. P. ; Litt, M. C. ; Mitchell, M. C. ; Sylvester, J. T. / Energy state and vasomotor tone in hypoxic pig lungs. In: Journal of Applied Physiology. 1991 ; Vol. 70, No. 4. pp. 1874-1881.
@article{42809f5fe8184239adc31f84f61c5941,
title = "Energy state and vasomotor tone in hypoxic pig lungs",
abstract = "To evaluate the role of energy state in pulmonary vascular responses to hypoxia, we exposed isolated pig lungs to decreases in inspired P(O2) or increases in perfusate NaCN concentration. Lung energy state was assessed by 31P nuclear magnetic resonance spectroscopy or measurement of adenine nucleotides by high-pressure liquid chromatography in freeze-clamped biopsies. In ventilated lungs, inspired P(O2) of 200 (normoxia), 50 (hypoxia), and 0 Torr (anoxia) did not change adenine nucleotides but resulted in steady-state pulmonary arterial pressure (Ppa) values of 15.5 ± 1.4, 30.3 ± 1.8, and 17.2 ± 1.9 mmHg, respectively, indicating vasoconstriction during hypoxia and reversal of vasoconstriction during anoxia. In degassed lungs, similar changes in Ppa were observed; however, energy state deteriorated during anoxia. An increase in perfusate NaCN concentration from 0 to 0.1 mM progressively increased Ppa and did not alter adenine nucleotides, whereas 1 mM reversed this vasoconstriction and caused deterioration of energy state. These results suggest that 1) pulmonary vasoconstrictor responses to hypoxia or cyanide occurred independently of whole lung energy state, 2) the inability of the pulmonary vasculature to sustain hypoxic vasoconstriction during anoxia might be associated with decreased energy state in some lung compartment, and 3) atelectasis was detrimental to whole lung energy state.",
keywords = "adenosine 5'-triphosphate, anoxia, cyanide, hypoxia, phosphorus-31 nuclear magnetic resonance spectroscopy, pulmonary arterial pressure",
author = "Philip Buescher and Pearse, {David B} and Pillai, {R. P.} and Litt, {M. C.} and Mitchell, {M. C.} and Sylvester, {J. T.}",
year = "1991",
language = "English (US)",
volume = "70",
pages = "1874--1881",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Energy state and vasomotor tone in hypoxic pig lungs

AU - Buescher, Philip

AU - Pearse, David B

AU - Pillai, R. P.

AU - Litt, M. C.

AU - Mitchell, M. C.

AU - Sylvester, J. T.

PY - 1991

Y1 - 1991

N2 - To evaluate the role of energy state in pulmonary vascular responses to hypoxia, we exposed isolated pig lungs to decreases in inspired P(O2) or increases in perfusate NaCN concentration. Lung energy state was assessed by 31P nuclear magnetic resonance spectroscopy or measurement of adenine nucleotides by high-pressure liquid chromatography in freeze-clamped biopsies. In ventilated lungs, inspired P(O2) of 200 (normoxia), 50 (hypoxia), and 0 Torr (anoxia) did not change adenine nucleotides but resulted in steady-state pulmonary arterial pressure (Ppa) values of 15.5 ± 1.4, 30.3 ± 1.8, and 17.2 ± 1.9 mmHg, respectively, indicating vasoconstriction during hypoxia and reversal of vasoconstriction during anoxia. In degassed lungs, similar changes in Ppa were observed; however, energy state deteriorated during anoxia. An increase in perfusate NaCN concentration from 0 to 0.1 mM progressively increased Ppa and did not alter adenine nucleotides, whereas 1 mM reversed this vasoconstriction and caused deterioration of energy state. These results suggest that 1) pulmonary vasoconstrictor responses to hypoxia or cyanide occurred independently of whole lung energy state, 2) the inability of the pulmonary vasculature to sustain hypoxic vasoconstriction during anoxia might be associated with decreased energy state in some lung compartment, and 3) atelectasis was detrimental to whole lung energy state.

AB - To evaluate the role of energy state in pulmonary vascular responses to hypoxia, we exposed isolated pig lungs to decreases in inspired P(O2) or increases in perfusate NaCN concentration. Lung energy state was assessed by 31P nuclear magnetic resonance spectroscopy or measurement of adenine nucleotides by high-pressure liquid chromatography in freeze-clamped biopsies. In ventilated lungs, inspired P(O2) of 200 (normoxia), 50 (hypoxia), and 0 Torr (anoxia) did not change adenine nucleotides but resulted in steady-state pulmonary arterial pressure (Ppa) values of 15.5 ± 1.4, 30.3 ± 1.8, and 17.2 ± 1.9 mmHg, respectively, indicating vasoconstriction during hypoxia and reversal of vasoconstriction during anoxia. In degassed lungs, similar changes in Ppa were observed; however, energy state deteriorated during anoxia. An increase in perfusate NaCN concentration from 0 to 0.1 mM progressively increased Ppa and did not alter adenine nucleotides, whereas 1 mM reversed this vasoconstriction and caused deterioration of energy state. These results suggest that 1) pulmonary vasoconstrictor responses to hypoxia or cyanide occurred independently of whole lung energy state, 2) the inability of the pulmonary vasculature to sustain hypoxic vasoconstriction during anoxia might be associated with decreased energy state in some lung compartment, and 3) atelectasis was detrimental to whole lung energy state.

KW - adenosine 5'-triphosphate

KW - anoxia

KW - cyanide

KW - hypoxia

KW - phosphorus-31 nuclear magnetic resonance spectroscopy

KW - pulmonary arterial pressure

UR - http://www.scopus.com/inward/record.url?scp=0025865055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025865055&partnerID=8YFLogxK

M3 - Article

C2 - 2055866

AN - SCOPUS:0025865055

VL - 70

SP - 1874

EP - 1881

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 4

ER -