Energy-linked anion transport. Cloning, sequencing, and characterization of a full length cDNA encoding the rat liver mitochondrial proton/phosphate symporter

G. C. Ferreira, R. D. Pratt, Peter L Pedersen

Research output: Contribution to journalArticle

Abstract

A full length cDNA clone encoding the precursor of the rat liver mitochondrial phosphate transporter (H+/P(i) symporter) has been isolated from a cDNA library using a bovine heart partial length phosphate transporter clone as a hybridization probe. The entire clone is 1263 base pairs in length with 5'- and 3'-untranslated regions of 16 and 168 base pairs, respectively. The open reading frame encodes for the mature protein (312 amino acids) preceded by a presequence of 44 amino acids enriched in basic residues. The polypeptide sequence predicted from the DNA sequence was confirmed by analyzing the first 17 amino-terminal amino acids of the pure phosphate transporter protein. The rat liver phosphate transporter differs from the bovine heart transporter in 32 amino acids (i.e. ~10%). It contains a region from amino acid 139 to 159 which is 37% identical with the β-subunit of the liver mitochondrial ATP synthase. Amino acid sequence comparisons of the P(i) transporter with P(i) binding proteins, other H+-linked symporters, and the human glucose transporter did not reveal significant sequence homology. Analysis of genomic DNA from both rat and S. cerevisiae by Southern blots using the rat liver mitochondrial P(i) carrier cDNA as a probe revealed remarkably similar restriction patterns, a finding consistent with the presence in lower and higher eukaryotes of homologous P(i) carrier proteins. This is the first report of the isolation, sequencing, and characterization of a full length cDNA coding for a protein involved in energy-coupled P(i) transport.

Original languageEnglish (US)
Pages (from-to)15628-15633
Number of pages6
JournalJournal of Biological Chemistry
Volume264
Issue number26
StatePublished - 1989

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Proton-Phosphate Symporters
Phosphate Transport Proteins
Cloning
Liver
Anions
Rats
Organism Cloning
Complementary DNA
Amino Acids
Symporters
Clone Cells
Base Pairing
Carrier Proteins
Mitochondrial Proton-Translocating ATPases
Proteins
Facilitative Glucose Transport Proteins
5' Untranslated Regions
3' Untranslated Regions
Sequence Homology
Southern Blotting

ASJC Scopus subject areas

  • Biochemistry

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Energy-linked anion transport. Cloning, sequencing, and characterization of a full length cDNA encoding the rat liver mitochondrial proton/phosphate symporter. / Ferreira, G. C.; Pratt, R. D.; Pedersen, Peter L.

In: Journal of Biological Chemistry, Vol. 264, No. 26, 1989, p. 15628-15633.

Research output: Contribution to journalArticle

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