Endotoxin inhibitor prevents sepsis-induced alterations in intestinal ion transport

Edward E. Whang, James C. Dunn, Harish Mahanty, David W. McFadden, Michael J. Zinner, Stanley W. Ashley

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The intestine is a target of septic insult. The aims of this study were to characterize sepsis-induced alterations in intestinal ion transport and to determine the role endotoxin plays in mediating these changes. METHODS: Rats underwent cecal manipulation alone (control), cecal ligation and puncture (CLP), or CLP plus intraperitoneal injection of 0.2 mg of a recently synthesized endotoxin inhibitor. At 24 hours, distal ileum was harvested, and transport parameters were determined. RESULTS: Cecal ligation and puncture produced a significant increase in short-circuit current (I(sc)) that was attributable to the induction of chloride secretion. There were no alterations in transepithelial resistance or fluxes of mannitol and sodium. The sepsis-induced increase in I(sc) was prevented by administration of the endotoxin inhibitor. CONCLUSIONS: In this model of sepsis, the primary alteration in ileal ion transport is an induction of electrogenic chloride secretion. Endotoxin inhibition may represent a strategy for prophylaxis against the intestinal effects of sepsis.

Original languageEnglish (US)
Pages (from-to)341-344
Number of pages4
JournalAmerican journal of surgery
Volume172
Issue number4
DOIs
StatePublished - Oct 1996

ASJC Scopus subject areas

  • Surgery

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    Whang, E. E., Dunn, J. C., Mahanty, H., McFadden, D. W., Zinner, M. J., & Ashley, S. W. (1996). Endotoxin inhibitor prevents sepsis-induced alterations in intestinal ion transport. American journal of surgery, 172(4), 341-344. https://doi.org/10.1016/S0002-9610(96)00189-4