Endothelium-derived contracting and relaxing factors contribute to hypoxic responses of pulmonary arteries

K. L. Kovitz, T. D. Aleskowitch, J. T. Sylvester, Nicholas Flavahan

Research output: Contribution to journalArticle

Abstract

The response of porcine pulmonary arteries to hypoxia depended on their location in the vasculature and the degree and duration of the hypoxic challenge. In rings of pulmonary artery suspended for isometric tension recording (37°C, 16% O2 and 5% CO2), moderate hypoxia (10% and 4% O2) caused endothelium-dependent relaxation in distal arteries but transient endothelium-dependent contraction in proximal arteries. In both proximal and distal arteries, the initial response to anoxia (0% O2) was a transient endothelium-dependent contraction. This was followed by a slowly developing, sustained endothelium-dependent contraction in proximal arteries, or by an endothelium-independent relaxation in distal arteries. The endothelium- dependent relaxation to moderate hypoxia in distal arteries was inhibited only by combined inhibition of endothelium-derived relaxing factor (EDRF)- nitric oxide (NO) synthase [N(ω)-nitro-L-arginine methyl ester (L-NAME)] and cyclooxygenase (indomethacin), suggesting mediation by EDRF-NO and prostacyclin. Transient endothelium-dependent contractions to moderate hypoxia (proximal arteries) or anoxia (all arteries) were abolished by L- NAME, but the late endothelium-dependent anoxic contraction observed in proximal arteries was not reduced by L-NAME and/or indomethacin. Therefore, hypoxia/anoxia may initiate contraction of pulmonary arteries by decreasing the activity of EDRF-NO, but the contractions appear to be maintained by an increased activity of an endothelium-derived contracting factor.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume265
Issue number4 34-4
StatePublished - 1993

Fingerprint

Endothelium-Dependent Relaxing Factors
pulmonary artery
endothelium
Pulmonary Artery
Endothelium
Arteries
arteries
hypoxia
NG-Nitroarginine Methyl Ester
Indomethacin
indomethacin
Nitric Oxide
nitric oxide
Hypoxia
Epoprostenol
Prostaglandin-Endoperoxide Synthases
prostacyclin
Nitric Oxide Synthase
prostaglandin synthase
Swine

Keywords

  • hypoxic pulmonary vasoconstriction
  • nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Endothelium-derived contracting and relaxing factors contribute to hypoxic responses of pulmonary arteries. / Kovitz, K. L.; Aleskowitch, T. D.; Sylvester, J. T.; Flavahan, Nicholas.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 265, No. 4 34-4, 1993.

Research output: Contribution to journalArticle

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