Endothelium-dependent relaxation is independent of arachidonic acid release

P. G. Milner, N. J. Izzo, J. A. Saye, A. L. Loeb, R. A. Johns, M. J. Peach

Research output: Contribution to journalArticlepeer-review

Abstract

Endothelium-dependent relaxation is mediated by the release from vascular endothelium of an endothelium-drived relaxing factor (EDRF). It is not clear what role arachidonic acid has in this process. Inhibition of phospholipase A2, and diacylglycerol lipase in cultured bovine aortic endothelial cells caused a marked reduction in agonist-induced arachidonic acid release from membrane phospholipid pools, and complete inhibition of prostacyclin production. EDRF release, assayed by measuring endothelium-dependent cGMP changes in mixed endothelial-smooth muscle cell cultures, was not inhibited under this conditions. In fact, EDRF release in response to two agonists, melittin and ATP, was actually increased in cells treated with phospholipase A2 inhibitors. In addition, pretreatment of rats with high-dose dexamethasone, an inhibitor of PLA2, did not attenuate endothelium-dependent relaxation in intact aortic rings removed from the animals, or depressor responses in anesthetized animals induced by endothelium-dependent vasodilators. In summary, inhibition of arachidonic acid release from membrane phospholipid pools does not attenuate endothelium-dependent relaxation in rats, or the release and/or response to EDRF in cultured cells.

Original languageEnglish (US)
Pages (from-to)1795-1803
Number of pages9
JournalJournal of Clinical Investigation
Volume81
Issue number6
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Endothelium-dependent relaxation is independent of arachidonic acid release'. Together they form a unique fingerprint.

Cite this