Endothelin receptor: a profoundly desensitizing receptor expressed in Xenopus oocytes

Shoichi Shimada, Charles Spivak, George Uhl

Research output: Contribution to journalArticlepeer-review


Xenopus oocyte expression studies can help to define the physiological properties of a receptor and can aid in receptor cloning. Expression of an endothelin receptor in oocytes injected with brain mRNA is inferred from depolarizations induced by several endothelin-related peptides. The response is abolished by intracellular EGTA injection but not in the absence of extracellular Ca2+. It is not present in non-injected oocytes, and reverses at a potential indicating that it is mediated by an increased Cl-1 conductance. Responses display striking, long-lasting desensitization. The expressed endothelin receptor thus displays properties characteristic of several receptors coupled to changes in phosphoinositide turnover, several of which have been successfully cloned using this response as a reporter.

Original languageEnglish (US)
Pages (from-to)123-125
Number of pages3
JournalEuropean Journal of Pharmacology
Issue number1
StatePublished - Jan 25 1991


  • (Receptor)
  • Brain
  • Desensitization
  • Endothelin
  • Sarafotoxin
  • Xenopus oocytes

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology


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