Endothelial cells create a stem cell niche in glioblastoma by providing NOTCH ligands that nurture self-renewal of cancer stem-like cells

Thant S. Zhu, Mark A. Costello, Caroline E. Talsma, Callie G. Flack, Jessica G. Crowley, Lisa L. Hamm, Xiaobing He, Shawn L. Hervey-Jumper, Jason A. Heth, Karin M. Muraszko, Francesco DiMeco, Angelo L. Vescovi, Xing Fan

Research output: Contribution to journalArticle

Abstract

One important function of endothelial cells in glioblastoma multiforme (GBM) is to create a niche that helps promote self-renewal of cancer stem-like cells (CSLC). However, the underlying molecular mechanism for this endothelial function is not known. Since activation of NOTCH signaling has been found to be required for propagation of GBM CSLCs, we hypothesized that the GBM endothelium may provide the source of NOTCH ligands. Here, we report a corroboration of this concept with a demonstration that NOTCH ligands are expressed in endothelial cells adjacent to NESTIN and NOTCH receptor-positive cancer cells in primary GBMs. Coculturing human brain microvascular endothelial cells (hBMEC) or NOTCH ligand with GBM neurospheres promoted GBM cell growth and increased CSLC self-renewal. Notably, RNAi-mediated knockdown of NOTCH ligands in hBMECs abrogated their ability to induce CSLC self-renewal and GBM tumor growth, both in vitro and in vivo. Thus, our findings establish that NOTCH activation in GBM CSLCs is driven by juxtacrine signaling between tumor cells and their surrounding endothelial cells in the tumor microenvironment, suggesting that targeting both CSLCs and their niche may provide a novel strategy to deplete CSLCs and improve GBM treatment.

Original languageEnglish (US)
Pages (from-to)6061-6072
Number of pages12
JournalCancer Research
Volume71
Issue number18
DOIs
StatePublished - Sep 15 2011

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Zhu, T. S., Costello, M. A., Talsma, C. E., Flack, C. G., Crowley, J. G., Hamm, L. L., He, X., Hervey-Jumper, S. L., Heth, J. A., Muraszko, K. M., DiMeco, F., Vescovi, A. L., & Fan, X. (2011). Endothelial cells create a stem cell niche in glioblastoma by providing NOTCH ligands that nurture self-renewal of cancer stem-like cells. Cancer Research, 71(18), 6061-6072. https://doi.org/10.1158/0008-5472.CAN-10-4269