Endothelial cell apoptosis is inhibited by a soluble factor secreted by human colon cancer cells

Wenbiao Liu, Darren W. Davis, Karen Ramirez, David McConkey, Lee M. Ellis

Research output: Contribution to journalArticle

Abstract

In contrast to normal tissues, tumors are exposed to adverse environmental conditions, such as hypoxia and acidity. Despite this caustic environment, tumor cells and supporting vascular structures survive the latter, providing nutrients and oxygen to facilitate tumor growth. Therefore, we hypothesized that cancer cells express factors that protect endothelial cells from apoptosis. Human umbilical vein endothelial cells were grown in serum-free medium or in medium conditioned by either human colon cancer cells or non-malignant cells. Endothelial cells grown in medium conditioned by colon cancer cells demonstrated a decrease in apoptosis, whereas endothelial cells grown in medium conditioned by non-malignant cells did not. Erk-1/2 phosphorylation increased when endothelial cells were incubated in medium conditioned by colon cancer cells but not when cells were incubated in medium conditioned by non-malignant cells. Protein fractions from medium conditioned by colon cancer cells that were <3 kDa protected endothelial cells from apoptosis and activated Erk-1/2. Heat-inactivated conditioned media did not protect endothelial cells from apoptosis and did not activate Erk-1/2, Human colon cancer cells secrete a soluble factor or factors that inhibit endothelial cell apoptosis. This factor is likely to be a protein or protein fragment because it loses its activity after heat inactivation. These studies support the hypothesis that tumor cells can alter the phenotype of endothelial cells.

Original languageEnglish (US)
Pages (from-to)26-30
Number of pages5
JournalInternational Journal of Cancer
Volume92
Issue number1
DOIs
StatePublished - Apr 1 2001
Externally publishedYes

Fingerprint

Colonic Neoplasms
Endothelial Cells
Apoptosis
Conditioned Culture Medium
Neoplasms
Hot Temperature
Caustics
Proteins
Serum-Free Culture Media
Human Umbilical Vein Endothelial Cells
Blood Vessels
Phosphorylation
Oxygen
Phenotype
Food

Keywords

  • Angiogenesis
  • Apoptosis
  • Colon carcinoma
  • Endothelial cells
  • Signal transduction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Endothelial cell apoptosis is inhibited by a soluble factor secreted by human colon cancer cells. / Liu, Wenbiao; Davis, Darren W.; Ramirez, Karen; McConkey, David; Ellis, Lee M.

In: International Journal of Cancer, Vol. 92, No. 1, 01.04.2001, p. 26-30.

Research output: Contribution to journalArticle

Liu, Wenbiao ; Davis, Darren W. ; Ramirez, Karen ; McConkey, David ; Ellis, Lee M. / Endothelial cell apoptosis is inhibited by a soluble factor secreted by human colon cancer cells. In: International Journal of Cancer. 2001 ; Vol. 92, No. 1. pp. 26-30.
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