Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo

Gopal Thinakaran; David R. Borchelt; Michael K. Lee; Hilda H. Slunt; Lia Spitzer; Grace Kim; Tamara Ratovitsky; Frances Davenport; Christer Nordstedt; Mary Seeger; John Hardy; Allan I. Levey; Samuel E. Gandy; Nancy A. Jenkins; Neal G. Copeland; Donald L. Price; Sangram S. Sisodia

doi:10.1016/S0896-6273(00)80291-3

Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo

Gopal Thinakaran, David R. Borchelt, Michael K. Lee, Hilda H. Slunt, Lia Spitzer, Grace Kim, Tamara Ratovitsky, Frances Davenport, Christer Nordstedt, Mary Seeger, John Hardy, Allan I. Levey, Samuel E. Gandy, Nancy A. Jenkins, Neal G. Copeland, Donald L. Price, Sangram S. Sisodia

School of Medicine

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915 Scopus citations

Abstract

The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are ~27-28 kDa N-terminal and ~16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, ~17 kDa and ~27 kDa PS1 derivatives accumulate to saturable levels, and at ~1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo.

Original language	English (US)
Pages (from-to)	181-190
Number of pages	10
Journal	Neuron
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/S0896-6273(00)80291-3
State	Published - Jul 1996

ASJC Scopus subject areas

General Neuroscience

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Thinakaran, G., Borchelt, D. R., Lee, M. K., Slunt, H. H., Spitzer, L., Kim, G., Ratovitsky, T., Davenport, F., Nordstedt, C., Seeger, M., Hardy, J., Levey, A. I., Gandy, S. E., Jenkins, N. A., Copeland, N. G., Price, D. L., & Sisodia, S. S. (1996). Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo. Neuron, 17(1), 181-190. https://doi.org/10.1016/S0896-6273(00)80291-3

Thinakaran, G, Borchelt, DR, Lee, MK, Slunt, HH, Spitzer, L, Kim, G, Ratovitsky, T, Davenport, F, Nordstedt, C, Seeger, M, Hardy, J, Levey, AI, Gandy, SE, Jenkins, NA, Copeland, NG, Price, DL & Sisodia, SS 1996, 'Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo', Neuron, vol. 17, no. 1, pp. 181-190. https://doi.org/10.1016/S0896-6273(00)80291-3

@article{140bebf04bd446cbbf7a31f184da9e1d,

title = "Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo",

abstract = "The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are ~27-28 kDa N-terminal and ~16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, ~17 kDa and ~27 kDa PS1 derivatives accumulate to saturable levels, and at ~1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo.",

author = "Gopal Thinakaran and Borchelt, {David R.} and Lee, {Michael K.} and Slunt, {Hilda H.} and Lia Spitzer and Grace Kim and Tamara Ratovitsky and Frances Davenport and Christer Nordstedt and Mary Seeger and John Hardy and Levey, {Allan I.} and Gandy, {Samuel E.} and Jenkins, {Nancy A.} and Copeland, {Neal G.} and Price, {Donald L.} and Sisodia, {Sangram S.}",

note = "Funding Information: This work was supported by National Institutes of Health grants AG0514, NS20471, and AG05689 to M. S. S., United States Public Health Service grants AG11508 and AG09464 to S. E. G., the National Cancer Institute, Department of Health and Human Services, under contract with Advanced BioScience Laboratories, and by grants from the Adler Foundation and Develbiss Fund. S. S. S. is the recipient of an Alzheimer's Association Zenith Award. ",

year = "1996",

month = jul,

doi = "10.1016/S0896-6273(00)80291-3",

language = "English (US)",

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pages = "181--190",

journal = "Neuron",

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T1 - Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo

AU - Thinakaran, Gopal

AU - Borchelt, David R.

AU - Lee, Michael K.

AU - Slunt, Hilda H.

AU - Spitzer, Lia

AU - Kim, Grace

AU - Ratovitsky, Tamara

AU - Davenport, Frances

AU - Nordstedt, Christer

AU - Seeger, Mary

AU - Hardy, John

AU - Levey, Allan I.

AU - Gandy, Samuel E.

AU - Jenkins, Nancy A.

AU - Copeland, Neal G.

AU - Price, Donald L.

AU - Sisodia, Sangram S.

N1 - Funding Information: This work was supported by National Institutes of Health grants AG0514, NS20471, and AG05689 to M. S. S., United States Public Health Service grants AG11508 and AG09464 to S. E. G., the National Cancer Institute, Department of Health and Human Services, under contract with Advanced BioScience Laboratories, and by grants from the Adler Foundation and Develbiss Fund. S. S. S. is the recipient of an Alzheimer's Association Zenith Award.

PY - 1996/7

Y1 - 1996/7

N2 - The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are ~27-28 kDa N-terminal and ~16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, ~17 kDa and ~27 kDa PS1 derivatives accumulate to saturable levels, and at ~1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo.

AB - The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are ~27-28 kDa N-terminal and ~16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, ~17 kDa and ~27 kDa PS1 derivatives accumulate to saturable levels, and at ~1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo.

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Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo

Abstract

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