Endoplasmic reticulum stress proteins are activated in rd retinal degeneration

Le Meng Wu, Mark Tso, Xiu An Zhu, Xiu Juan Guo, Li Ping Yang

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the expression of endoplasmic reticulum (ER) stress proteins in photoreceptor apoptosis in rd mouse (Pde6bRd1/Rd1). Methods: Photoreceptor apoptosis in rd mouse was detected by terminal dUTP transferase nick end labeling (TUNEL). The protein expression of ER stress sensors including glucose-regulated protein-78 (GRP78/BiP), caspase-12, phospho-eukaryotic initiation factor 2α (eIF2α) and phospho-pancreatic ER kinase (PERK) was examined by immunoflurescence and Western Blot analysis. Results: Accompanying photoreceptor apoptosis in rd mouse, protein expression of GRP78/BiP, caspase-12, phospho-eIF2α and phospho-PERK was up-regulated in a time dependent manner. The up-regulation of these proteins coincided with or preceded the photoreceptor apoptosis. At the peak of their expression, they were mainly located in the photoreceptor inner segment and/or outer nuclear layer (ONL). Conclusion: Activation of ER stress proteins appears to play an important role in rd retinal degeneration. Therefore endoplasmic reticulum stress modulators could be a strong candidate as a therapeutic agent in the treatment of these diseases.

Original languageEnglish (US)
Pages (from-to)807-812
Number of pages6
JournalChinese Journal of Ophthalmology
Volume44
Issue number9
StatePublished - Sep 1 2008
Externally publishedYes

Keywords

  • Apoptosis
  • Endoplasmic reticulum
  • Heat-shock proteins
  • Mice
  • Retinal degeneration

ASJC Scopus subject areas

  • Ophthalmology

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