Endogenous histamine excites neurones in the guinea-pig superior cervical ganglion in vitro

E. P. Christian, Bradley J Undem, D. Weinreich

Research output: Contribution to journalArticle

Abstract

Intracellular recordings were obtained from neurones in the guinea-pig superior cervical ganglion (SCG) in vitro to study the electrophysiological effects of endogenously released histamine. Guinea-pigs were actively sensitized to the specific antigen, ovalbumin. SCG removed from these animals rapidly released a significant proportion of their endogenous histamine stores into the extracellular space upon exposure to the sensitizing antigen. Several observations indicated that the released histamine was derived from ganglionic mast cells. The electrophysiological effects produced by antigen challenge in a neurone mimicked qualitatively and quantitatively those effects produced by exogenously applied histamine in the same neurone. Under current clamp the membrane effects of antigen and histamine included a transient depolarization, an increase in input resistance and transient blockade of a long-duration component of the spike after-hyperpolarization. In voltage clamp histamine and antigen produced an inward current and decreased membrane conductance. Histamine H1, but not H2 or H3 receptor antagonists prevented the membrane depolarization to both histamine and antigen treatments. These convergent biochemical, physiological and pharmacological data demonstrate that a sufficient quantity of endogenous histamine is released by an antigenic stimulus in SCG from sensitized guinea-pigs to affect specific electrophysiological characteristics of neurones. Histamine may thus be involved in mediating interactions between the mammalian immune system and the peripheral sympathetic nervous system.

Original languageEnglish (US)
Pages (from-to)297-312
Number of pages16
JournalJournal of Physiology
Volume409
StatePublished - 1989
Externally publishedYes

Fingerprint

Superior Cervical Ganglion
Histamine
Guinea Pigs
Neurons
Antigens
Membranes
In Vitro Techniques
Histamine H3 Receptors
Histamine H2 Receptors
Sympathetic Nervous System
Ovalbumin
Peripheral Nervous System
Extracellular Space
Mast Cells
Immune System
Pharmacology

ASJC Scopus subject areas

  • Physiology

Cite this

Endogenous histamine excites neurones in the guinea-pig superior cervical ganglion in vitro. / Christian, E. P.; Undem, Bradley J; Weinreich, D.

In: Journal of Physiology, Vol. 409, 1989, p. 297-312.

Research output: Contribution to journalArticle

@article{251ffd39f38948f480105ec51357e60c,
title = "Endogenous histamine excites neurones in the guinea-pig superior cervical ganglion in vitro",
abstract = "Intracellular recordings were obtained from neurones in the guinea-pig superior cervical ganglion (SCG) in vitro to study the electrophysiological effects of endogenously released histamine. Guinea-pigs were actively sensitized to the specific antigen, ovalbumin. SCG removed from these animals rapidly released a significant proportion of their endogenous histamine stores into the extracellular space upon exposure to the sensitizing antigen. Several observations indicated that the released histamine was derived from ganglionic mast cells. The electrophysiological effects produced by antigen challenge in a neurone mimicked qualitatively and quantitatively those effects produced by exogenously applied histamine in the same neurone. Under current clamp the membrane effects of antigen and histamine included a transient depolarization, an increase in input resistance and transient blockade of a long-duration component of the spike after-hyperpolarization. In voltage clamp histamine and antigen produced an inward current and decreased membrane conductance. Histamine H1, but not H2 or H3 receptor antagonists prevented the membrane depolarization to both histamine and antigen treatments. These convergent biochemical, physiological and pharmacological data demonstrate that a sufficient quantity of endogenous histamine is released by an antigenic stimulus in SCG from sensitized guinea-pigs to affect specific electrophysiological characteristics of neurones. Histamine may thus be involved in mediating interactions between the mammalian immune system and the peripheral sympathetic nervous system.",
author = "Christian, {E. P.} and Undem, {Bradley J} and D. Weinreich",
year = "1989",
language = "English (US)",
volume = "409",
pages = "297--312",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Endogenous histamine excites neurones in the guinea-pig superior cervical ganglion in vitro

AU - Christian, E. P.

AU - Undem, Bradley J

AU - Weinreich, D.

PY - 1989

Y1 - 1989

N2 - Intracellular recordings were obtained from neurones in the guinea-pig superior cervical ganglion (SCG) in vitro to study the electrophysiological effects of endogenously released histamine. Guinea-pigs were actively sensitized to the specific antigen, ovalbumin. SCG removed from these animals rapidly released a significant proportion of their endogenous histamine stores into the extracellular space upon exposure to the sensitizing antigen. Several observations indicated that the released histamine was derived from ganglionic mast cells. The electrophysiological effects produced by antigen challenge in a neurone mimicked qualitatively and quantitatively those effects produced by exogenously applied histamine in the same neurone. Under current clamp the membrane effects of antigen and histamine included a transient depolarization, an increase in input resistance and transient blockade of a long-duration component of the spike after-hyperpolarization. In voltage clamp histamine and antigen produced an inward current and decreased membrane conductance. Histamine H1, but not H2 or H3 receptor antagonists prevented the membrane depolarization to both histamine and antigen treatments. These convergent biochemical, physiological and pharmacological data demonstrate that a sufficient quantity of endogenous histamine is released by an antigenic stimulus in SCG from sensitized guinea-pigs to affect specific electrophysiological characteristics of neurones. Histamine may thus be involved in mediating interactions between the mammalian immune system and the peripheral sympathetic nervous system.

AB - Intracellular recordings were obtained from neurones in the guinea-pig superior cervical ganglion (SCG) in vitro to study the electrophysiological effects of endogenously released histamine. Guinea-pigs were actively sensitized to the specific antigen, ovalbumin. SCG removed from these animals rapidly released a significant proportion of their endogenous histamine stores into the extracellular space upon exposure to the sensitizing antigen. Several observations indicated that the released histamine was derived from ganglionic mast cells. The electrophysiological effects produced by antigen challenge in a neurone mimicked qualitatively and quantitatively those effects produced by exogenously applied histamine in the same neurone. Under current clamp the membrane effects of antigen and histamine included a transient depolarization, an increase in input resistance and transient blockade of a long-duration component of the spike after-hyperpolarization. In voltage clamp histamine and antigen produced an inward current and decreased membrane conductance. Histamine H1, but not H2 or H3 receptor antagonists prevented the membrane depolarization to both histamine and antigen treatments. These convergent biochemical, physiological and pharmacological data demonstrate that a sufficient quantity of endogenous histamine is released by an antigenic stimulus in SCG from sensitized guinea-pigs to affect specific electrophysiological characteristics of neurones. Histamine may thus be involved in mediating interactions between the mammalian immune system and the peripheral sympathetic nervous system.

UR - http://www.scopus.com/inward/record.url?scp=0024578586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024578586&partnerID=8YFLogxK

M3 - Article

C2 - 2585292

AN - SCOPUS:0024578586

VL - 409

SP - 297

EP - 312

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

ER -