Endogenous β-galactosidase activity in continuously nonproliferating cells

Yegor E. Yegorov, Sergey Akimov, Ralf Hass, Alexander V. Zelenin, Igor A. Prudovsky

Research output: Contribution to journalArticle

Abstract

Cytochemically detectable activity of endogenous β-galactosidase was found at pH 6.0 in Swiss 3T3 cells after long-term incubation in low serum or in the presence of heparin concentrations known to reversibly inhibit cell proliferation. A high percentage of β-galactosidase-positive cells were detected in U937 and HL60 cultures at the late stage of macrophage-like differentiation induced by TPA. Interestingly, a small number of β- galactosidase-positive cells were found even in the growing Swiss 3T3 cultures. These positive cells expressed morphological features similar to those of senescent cells. Thus, the activity of β-galactosidase at pH 6.0 cannot be considered an exclusive marker of senescent cells since it is expressed in other types of nonproliferating cells.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalExperimental Cell Research
Volume243
Issue number1
DOIs
StatePublished - Aug 25 1998
Externally publishedYes

Fingerprint

Galactosidases
Swiss 3T3 Cells
Heparin
Macrophages
Cell Proliferation
Serum

Keywords

  • β- galactosidase
  • Differentiation
  • Fibroblasts
  • Leukemia
  • Quiescence
  • Senescence

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endogenous β-galactosidase activity in continuously nonproliferating cells. / Yegorov, Yegor E.; Akimov, Sergey; Hass, Ralf; Zelenin, Alexander V.; Prudovsky, Igor A.

In: Experimental Cell Research, Vol. 243, No. 1, 25.08.1998, p. 207-211.

Research output: Contribution to journalArticle

Yegorov, Yegor E. ; Akimov, Sergey ; Hass, Ralf ; Zelenin, Alexander V. ; Prudovsky, Igor A. / Endogenous β-galactosidase activity in continuously nonproliferating cells. In: Experimental Cell Research. 1998 ; Vol. 243, No. 1. pp. 207-211.
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