Endogenous β-galactosidase activity in continuously nonproliferating cells

Yegor E. Yegorov, Sergey S. Akimov, Ralf Hass, Alexander V. Zelenin, Igor A. Prudovsky

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Cytochemically detectable activity of endogenous β-galactosidase was found at pH 6.0 in Swiss 3T3 cells after long-term incubation in low serum or in the presence of heparin concentrations known to reversibly inhibit cell proliferation. A high percentage of β-galactosidase-positive cells were detected in U937 and HL60 cultures at the late stage of macrophage-like differentiation induced by TPA. Interestingly, a small number of β- galactosidase-positive cells were found even in the growing Swiss 3T3 cultures. These positive cells expressed morphological features similar to those of senescent cells. Thus, the activity of β-galactosidase at pH 6.0 cannot be considered an exclusive marker of senescent cells since it is expressed in other types of nonproliferating cells.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalExperimental cell research
Issue number1
StatePublished - Aug 25 1998
Externally publishedYes


  • Differentiation
  • Fibroblasts
  • Leukemia
  • Quiescence
  • Senescence
  • β- galactosidase

ASJC Scopus subject areas

  • Cell Biology


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