Endoderm-secreted factor stimulates growth of embryonal carcinoma stem cells

Emily L. Germain, John W. Littlefield

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Stem cells of the embryonal carcinoma cell line called H6 can be induced to differnetiate to endoderm-like cells by retinoic acid (3×10-6M). We have detected a diffusible and stable factor which is secreted by H6 endoderm-like cells and stimulates the growth of H6 stem cells. The stimulation by the endoderm-like cells is considereably greater than that by mouse fibroblasts or H6 stem cells themselves. No reciprocal stimulation of endoderm-like cells by stem cells occurs. Part but not all of the stimulation might be due to extracellular matrix proteins or to insulin-like growth factor type 2, each of which also stimulates the growth of H6 stem cells. Insulin causes no such stimulation.

Original languageEnglish (US)
Pages (from-to)107-112
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume22
Issue number2
DOIs
StatePublished - Feb 1986
Externally publishedYes

Keywords

  • embryonal carcinoma
  • growth factor

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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