Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical Polarity

Eugenio Marco, Roland Wedlich-Soldner, Rong Li, Steven J. Altschuler, Lani F. Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Diverse cell types require the ability to maintain dynamically polarized membrane-protein distributions through balancing transport and diffusion. However, design principles underlying dynamically maintained cortical polarity are not well understood. Here we constructed a mathematical model for characterizing the morphology of dynamically polarized protein distributions. We developed analytical approaches for measuring all model parameters from single-cell experiments. We applied our methods to a well-characterized system for studying polarized membrane proteins: budding yeast cells expressing activated Cdc42. We found that a balance of diffusion, directed transport, and endocytosis was sufficient for accurately describing polarization morphologies. Surprisingly, the model predicts that polarized regions are defined with a precision that is nearly optimal for measured endocytosis rates and that polarity can be dynamically stabilized through positive feedback with directed transport. Our approach provides a step toward understanding how biological systems shape spatially precise, unambiguous cortical polarity domains using dynamic processes.

Original languageEnglish (US)
Pages (from-to)411-422
Number of pages12
JournalCell
Volume129
Issue number2
DOIs
StatePublished - Apr 20 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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