Endocytosis and dissociation of class I MHC molecules labeled with fluorescent β-2 microglobulin

J. H. Hochman, H. Jiang, L. Matyus, M. Edidin, B. Pernis

Research output: Contribution to journalArticle

Abstract

Membrane class I MHC molecules of Con-A activated and lymphoma murine cells have been labeled by exchange of the cell's β2m with soluble fl-β2m. It has previously been shown that this method of labeling is specific and does not affect the biologic properties of class I MHC Ag. With this labeling it has been possible to demonstrate the constitutive endocytosis of class I MHC by fluorescence microscopy and by measuring the resistance to quenching by crystal violet of the internalized fl-β2m molecules. We could also follow the kinetics of β2m dissociation from the class I molecules at different pH. At pH 5.5, that is the average pH of endosomes, there is considerable dissociation within 15 to 20 min, that is the average recycling half time of class I MHC containing endosomes in activated T cells. Inasmuch as the process is reversible it is likely that, in the recycling endosomes of T cells, class I MHC molecules undergo conformational changes with β2m going off and on and with consequent changes of the peptide binding site. This process might be involved in Ag presentation, but, because it is apparently limited to T cells, it would play a role in the presentation of the cell's own TCR in idiotypic interactions between T cells.

Original languageEnglish (US)
Pages (from-to)1862-1867
Number of pages6
JournalJournal of Immunology
Volume146
Issue number6
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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