Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition

Yumiko Nakajima; Yasushi Furuichi; Kamal Krishna Biswas; Teruto Hashiguchi; Ko Ichi Kawahara; Kazuyo Yamaji; Tomonori Uchimura; Yuichi Izumi; Ikuro Maruyama

doi:10.1016/j.febslet.2005.12.079

Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition

Yumiko Nakajima, Yasushi Furuichi, Kamal Krishna Biswas, Teruto Hashiguchi, Ko Ichi Kawahara, Kazuyo Yamaji, Tomonori Uchimura, Yuichi Izumi, Ikuro Maruyama

School of Medicine

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.

Original language	English (US)
Pages (from-to)	613-619
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2005.12.079
State	Published - Jan 23 2006

Keywords

Anandamide
Cannabinoid receptor
Cytokine
NF-κB
Periodontitis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2005.12.079

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title = "Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition",

abstract = "Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.",

keywords = "Anandamide, Cannabinoid receptor, Cytokine, NF-κB, Periodontitis",

author = "Yumiko Nakajima and Yasushi Furuichi and Biswas, {Kamal Krishna} and Teruto Hashiguchi and Kawahara, {Ko Ichi} and Kazuyo Yamaji and Tomonori Uchimura and Yuichi Izumi and Ikuro Maruyama",

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AU - Nakajima, Yumiko

AU - Furuichi, Yasushi

AU - Biswas, Kamal Krishna

AU - Hashiguchi, Teruto

AU - Kawahara, Ko Ichi

AU - Yamaji, Kazuyo

AU - Uchimura, Tomonori

AU - Izumi, Yuichi

AU - Maruyama, Ikuro

PY - 2006/1/23

Y1 - 2006/1/23

N2 - Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.

AB - Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.

KW - Anandamide

KW - Cannabinoid receptor

KW - Cytokine

KW - NF-κB

KW - Periodontitis

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ER -

Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition

Abstract

Keywords

ASJC Scopus subject areas

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