Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition

Yumiko Nakajima, Yasushi Furuichi, Kamal Krishna Biswas, Teruto Hashiguchi, Ko Ichi Kawahara, Kazuyo Yamaji, Tomonori Uchimura, Yuichi Izumi, Ikuro Maruyama

Research output: Contribution to journalArticlepeer-review

Abstract

Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.

Original languageEnglish (US)
Pages (from-to)613-619
Number of pages7
JournalFEBS Letters
Volume580
Issue number2
DOIs
StatePublished - Jan 23 2006

Keywords

  • Anandamide
  • Cannabinoid receptor
  • Cytokine
  • NF-κB
  • Periodontitis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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