Ena/VASP Is Required for Neuritogenesis in the Developing Cortex

Adam V. Kwiatkowski, Douglas A. Rubinson, Erik W. Dent, J. Edward van Veen, Jonathan D. Leslie, Jiangyang Zhang, Leslie M. Mebane, Ulrike Philippar, Elaine M. Pinheiro, Aurora A. Burds, Roderick T. Bronson, Susumu Mori, Reinhard Fässler, Frank B. Gertler

Research output: Contribution to journalArticlepeer-review

Abstract

Mammalian cortical development involves neuronal migration and neuritogenesis; this latter process forms the structural precursors to axons and dendrites. Elucidating the pathways that regulate the cytoskeleton to drive these processes is fundamental to our understanding of cortical development. Here we show that loss of all three murine Ena/VASP proteins, a family of actin regulatory proteins, causes neuronal ectopias, alters intralayer positioning in the cortical plate, and, surprisingly, blocks axon fiber tract formation during corticogenesis. Cortical fiber tract defects in the absence of Ena/VASP arise from a failure in neurite initiation, a prerequisite for axon formation. Neurite initiation defects in Ena/VASP-deficient neurons are preceded by a failure to form bundled actin filaments and filopodia. These findings provide insight into the regulation of neurite formation and the role of the actin cytoskeleton during cortical development.

Original languageEnglish (US)
Pages (from-to)441-455
Number of pages15
JournalNeuron
Volume56
Issue number3
DOIs
StatePublished - Nov 8 2007

Keywords

  • DEVBIO
  • MOLNEURO

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Ena/VASP Is Required for Neuritogenesis in the Developing Cortex'. Together they form a unique fingerprint.

Cite this