@article{4c66b1b157354d1aa7b3132783ace57f,
title = "Ena/VASP Is Required for Neuritogenesis in the Developing Cortex",
abstract = "Mammalian cortical development involves neuronal migration and neuritogenesis; this latter process forms the structural precursors to axons and dendrites. Elucidating the pathways that regulate the cytoskeleton to drive these processes is fundamental to our understanding of cortical development. Here we show that loss of all three murine Ena/VASP proteins, a family of actin regulatory proteins, causes neuronal ectopias, alters intralayer positioning in the cortical plate, and, surprisingly, blocks axon fiber tract formation during corticogenesis. Cortical fiber tract defects in the absence of Ena/VASP arise from a failure in neurite initiation, a prerequisite for axon formation. Neurite initiation defects in Ena/VASP-deficient neurons are preceded by a failure to form bundled actin filaments and filopodia. These findings provide insight into the regulation of neurite formation and the role of the actin cytoskeleton during cortical development.",
keywords = "DEVBIO, MOLNEURO",
author = "Kwiatkowski, {Adam V.} and Rubinson, {Douglas A.} and Dent, {Erik W.} and {Edward van Veen}, J. and Leslie, {Jonathan D.} and Jiangyang Zhang and Mebane, {Leslie M.} and Ulrike Philippar and Pinheiro, {Elaine M.} and Burds, {Aurora A.} and Bronson, {Roderick T.} and Susumu Mori and Reinhard F{\"a}ssler and Gertler, {Frank B.}",
note = "Funding Information: We are indebted to the MIT DCM staff and injection facility for generation and maintenance of mouse colonies and to Alicia Caron and the MIT CCR Histology facility for tissue preparation and histology work. We especially thank Zhigang Xie and Li-Huei Tsai for help with in utero electroporation. We thank Morgan Sheng and Edward Morrisey for reagents. We appreciate Chris Walsh, Carlos Lois, Louis Reichardt, Sue McConnell, Elizabeth Alcamo, Jeff Macklis, Mary B. Hatten, W. James Nelson, and present and former members of the Gertler Lab for their helpful comments and discussion. A.V.K. was supported by an Anna Fuller Predoctoral Fellowship. D.A.R. was supported by a Ludwig Fellowship. E.W.D. was supported by NIH grant F32-NS45366. This work was supported by funds from the Stanley Medical Research Institute to J.E.V. and F.B.G.; NIH grant GM58801 (F.B.G.); EB003543 and AG20012 (S.M); U54 GM064346 (to the Cell Migration Consortium for A.A.B and the generation of EVL mice and mmvvee ES cells); and the Max Planck Society (R.F.). ",
year = "2007",
month = nov,
day = "8",
doi = "10.1016/j.neuron.2007.09.008",
language = "English (US)",
volume = "56",
pages = "441--455",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}