TY - JOUR
T1 - Enabled and Capping protein play important roles in shaping cell behavior during Drosophila oogenesis
AU - Gates, Julie
AU - Nowotarski, Stephanie H.
AU - Yin, Hongyan
AU - Mahaffey, James P.
AU - Bridges, Tina
AU - Herrera, Cristina
AU - Homem, Catarina C.F.
AU - Janody, Florence
AU - Montell, Denise J.
AU - Peifer, Mark
N1 - Funding Information:
This work was supported by NIHGM47857 to MP and NIHGM73164 to DJM. JG was a Leukemia and Lymphoma Society Special Fellow. JG was supported by NIH5F32GM068337 and Bucknell University's Swanson Fellowship, SHN by Cell and Molecular Biology Training Grant T32 GM008581, JPM by the Smallwood Foundation and a Goldwater Scholarship, and MP by the Hooker Distinguished Professorship.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - During development, cells craft an impressive array of actin-based structures, mediating events as diverse as cytokinesis, apical constriction, and cell migration. One challenge is to determine how cells regulate actin assembly and disassembly to carry out these cell behaviors. During Drosophila oogenesis diverse cell behaviors are seen in the soma and germline. We used oogenesis to explore developmental roles of two important actin regulators: Enabled/VASP proteins and Capping protein. We found that Enabled plays an important role in cortical integrity of nurse cells, formation of robust bundled actin filaments in late nurse cells that facilitate nurse cell dumping, and migration of somatic border cells. During nurse cell dumping, Enabled localizes to barbed ends of the nurse cell actin filaments, suggesting its mechanism of action. We further pursued this mechanism using mutant Enabled proteins, each affecting one of its protein domains. These data suggest critical roles for the EVH2 domain and its tetramerization subdomain, while the EVH1 domain appears less critical. Enabled appears to be negatively regulated during oogenesis by Abelson kinase. We also explored the function of Capping protein. This revealed important roles in oocyte determination, nurse cell cortical integrity and nurse cell dumping, and support the idea that Capping protein and Enabled act antagonistically during dumping. Together these data reveal places that these actin regulators shape oogenesis.
AB - During development, cells craft an impressive array of actin-based structures, mediating events as diverse as cytokinesis, apical constriction, and cell migration. One challenge is to determine how cells regulate actin assembly and disassembly to carry out these cell behaviors. During Drosophila oogenesis diverse cell behaviors are seen in the soma and germline. We used oogenesis to explore developmental roles of two important actin regulators: Enabled/VASP proteins and Capping protein. We found that Enabled plays an important role in cortical integrity of nurse cells, formation of robust bundled actin filaments in late nurse cells that facilitate nurse cell dumping, and migration of somatic border cells. During nurse cell dumping, Enabled localizes to barbed ends of the nurse cell actin filaments, suggesting its mechanism of action. We further pursued this mechanism using mutant Enabled proteins, each affecting one of its protein domains. These data suggest critical roles for the EVH2 domain and its tetramerization subdomain, while the EVH1 domain appears less critical. Enabled appears to be negatively regulated during oogenesis by Abelson kinase. We also explored the function of Capping protein. This revealed important roles in oocyte determination, nurse cell cortical integrity and nurse cell dumping, and support the idea that Capping protein and Enabled act antagonistically during dumping. Together these data reveal places that these actin regulators shape oogenesis.
KW - Abelson kinase
KW - Actin
KW - Capping protein
KW - Drosophila
KW - Ena/VASP
KW - Oogenesis
UR - http://www.scopus.com/inward/record.url?scp=68349125409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68349125409&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2009.06.030
DO - 10.1016/j.ydbio.2009.06.030
M3 - Article
C2 - 19576200
AN - SCOPUS:68349125409
SN - 0012-1606
VL - 333
SP - 90
EP - 107
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -