Emergence and persistence of CXCR4-tropic HIV-1 in a population of men from the multicenter AIDS cohort study

James C. Shepherd, Lisa Paula Jacobson, Wei Qiao, Beth D. Jamieson, John P. Phair, Paolo Piazza, Thomas C Quinn, Joseph Bernard Margolick

Research output: Contribution to journalArticle

Abstract

We examined the emergence of CXCR4 (i.e., X4) tropism in 67 male human immunodeficiency virus type 1 (HIV-1) seroconverters from the Multicenter AIDS Cohort Study (MACS) who were selected to reflect the full spectrum of rates of HIV-1 disease progression. A mean of 10 serial samples per donor were evaluated by a laboratory-validated, commercially available assay to determine phenotypic coreceptor use. A total of 52% of men had dual- or mixed-tropic HIV-1 detected at 1 or more of the time points tested. Use of X4 by HIV-1 was detected more frequently among men who developed AIDS (defined as a CD4+ T cell count of <200 cells/μL and/or an AIDS-defining illness) ≤ 11 years after seroconversion than among those who did not (P = .005), as well as among men who exhibited a total T cell count decline (i.e., a CD3+ inflection point), compared with those who did not (P = .03). For men in whom both X4 virus and an inflection point were detected, emergence of X4 virus preceded the inflection point by a median of 0.83 years. The median CD4 + T cell count at first detection of X4 viruses before the onset of AIDS was 475 cells/μL. We conclude that HIV-1 variants that used X4 frequently emerged at high CD4+ T cell counts and may contribute to the decrease in T cell numbers during late HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)1104-1112
Number of pages9
JournalJournal of Infectious Diseases
Volume198
Issue number8
DOIs
StatePublished - Oct 15 2008

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HIV-1
Acquired Immunodeficiency Syndrome
Cohort Studies
T-Lymphocytes
CD4 Lymphocyte Count
Population
Virus Diseases
Viruses
Cell Count
Tropism
Disease Progression
Tissue Donors

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Emergence and persistence of CXCR4-tropic HIV-1 in a population of men from the multicenter AIDS cohort study. / Shepherd, James C.; Jacobson, Lisa Paula; Qiao, Wei; Jamieson, Beth D.; Phair, John P.; Piazza, Paolo; Quinn, Thomas C; Margolick, Joseph Bernard.

In: Journal of Infectious Diseases, Vol. 198, No. 8, 15.10.2008, p. 1104-1112.

Research output: Contribution to journalArticle

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