Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos

Hye Soo Kim, Gab Sang Lee, Sang Hwan Hyun, Dong Hyun Nam, So Hyun Lee, Yeon Woo Jeong, Sue Kim, Ji Hye Kim, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

The present study investigated the expression of receptors for glycosaminoglycans (GAGs) and the effect of GAGs supplementation on development of porcine IVF embryos. Total RNA was prepared from oocytes, 2-, 4- and 8-cell embryos, morulae and blastocysts. The expression of hyaluronic acid receptor (CD44) and heparin (HP) interacting protein (HIP) was determined using RT-PCR and Southern blot analysis. The CD44 and HIP mRNA were detected from in vitro matured oocytes and all stages of pre-implantation embryos. The IVF embryos were cultured in modified NCSU-23 medium supplemented with various concentrations (0, 0.1, 0.5 or 1.0 mg/mL) of hyaluronic acid (HA) or heparin. Supplementing with 0.5 mg/mL HA significantly increased total cell number compared to other experimental groups, due to increase in trophectoderm cells. Supplementing with 1.0 mg/mL, HP significantly increased blastocyst formation rate compared to the control group. Supplementing media, in which IVF embryos were cultured with 0.5 mg/mL HA + 1.0 mg/mL HP, significantly increased blastocyst formation rate compared to the control group. In conclusion, the present study demonstrated the expression of HA and HP receptors and the embryotrophic effect of HA or HP on porcine IVF embryos.

Original languageEnglish (US)
Pages (from-to)1167-1180
Number of pages14
JournalTheriogenology
Volume63
Issue number4
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Keywords

  • Cell number
  • Embryo development
  • GAGs
  • GAGs receptors
  • Porcine IVF

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Fingerprint

Dive into the research topics of 'Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos'. Together they form a unique fingerprint.

Cite this