ELISA for antibody to hepatitis E virus (HEV) based on complete open-reading frame-2 protein expressed in insect cells: Identification of HEV infection in primates

Sergei A. Tsarev, Tatiana S. Tsareva, Suzanne U. Emerson, Albert Z. Kapikian, John R Ticehurst, William London, Robert H. Purcell

Research output: Contribution to journalArticle

Abstract

A recombinant baculovirus containing the complete open-reading frame (ORF)-2 region of the hepatitis E virus (HEV) genome was constructed. The major protein synthesized in insect cells infected with recombinant virus was about the size expected for the complete ORF-2 product. This protein reacted in a Western blot assay with plasma from an HEV-infected chimpanzee. Lysates of the recombinant virus-infected insect cells were used in ELISA to monitor serocon-version of eight primate species (chimpanzees, four species of Old World monkeys, and three species of New World monkeys) inoculated with HEV. Homologous detector anti-immunoglobulin was more sensitive than heterologous anti-immunoglobulin for detecting anti-HEV by ELISA. All primate species except tamarins seroconverted after inoculation with HEV, although anti-HEV titers of Old World monkey species were generally higher than those of New World monkey species. The ELISA with complete ORF-2 antigen appeared to be a sensitive and practical method for detecting anti-HEV.

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalJournal of Infectious Diseases
Volume168
Issue number2
StatePublished - Aug 1993
Externally publishedYes

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Hepatitis E virus
Virus Diseases
Primates
Open Reading Frames
Insects
Enzyme-Linked Immunosorbent Assay
Antibodies
Platyrrhini
Proteins
Cercopithecidae
Pan troglodytes
Immunoglobulins
Insect Viruses
Baculoviridae
Viral Load
Western Blotting
Genome
Viruses
Antigens

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

ELISA for antibody to hepatitis E virus (HEV) based on complete open-reading frame-2 protein expressed in insect cells : Identification of HEV infection in primates. / Tsarev, Sergei A.; Tsareva, Tatiana S.; Emerson, Suzanne U.; Kapikian, Albert Z.; Ticehurst, John R; London, William; Purcell, Robert H.

In: Journal of Infectious Diseases, Vol. 168, No. 2, 08.1993, p. 369-378.

Research output: Contribution to journalArticle

Tsarev, Sergei A. ; Tsareva, Tatiana S. ; Emerson, Suzanne U. ; Kapikian, Albert Z. ; Ticehurst, John R ; London, William ; Purcell, Robert H. / ELISA for antibody to hepatitis E virus (HEV) based on complete open-reading frame-2 protein expressed in insect cells : Identification of HEV infection in primates. In: Journal of Infectious Diseases. 1993 ; Vol. 168, No. 2. pp. 369-378.
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