Elimination of mycoplasma from human B-lymphoblastoid cell lines

David N. Howell, Carolyn E. Machamer, Peter Cresswell

Research output: Contribution to journalArticlepeer-review

Abstract

Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all cases, nude mouse passage for as little as 5 days was totally effective in removing all contaminating mycoplasma.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalHuman Immunology
Volume5
Issue number3
DOIs
StatePublished - Nov 1982
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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