Elevations in cathepsin B protein content and enzyme activity occur independently of glycosylation during colorectal tumor progression

Christine A. Iacobuzio-Donahue, Sania Shuja, Jinguo Cai, Phyllis Peng, Mary Jo Murnane

Research output: Contribution to journalArticle

Abstract

Western blots, enzyme assays, protein glycosylation studies, and immunohistochemical staining were used to characterize cathepsin B expression at successive stages of colorectal tumor progression. In normal colon mucosa and premalignant adenomas, cathepsin B expression was predominantly due to mature two-chain protein detected on Western blots as the nonglycosylated 27- kDa form, with overexpression of this protein occurring in only 4 of 18 adenomas. Overexpression increased significantly in Dukes A and B carcinomas (26 of 37 cases), with cathepsin B protein generally detectable in carcinomas as a combination of both 27-kDa nonglycosylated and 28-kDa glycosylated mature two-chain forms. Glycosylated cathepsin B protein in carcinoma extracts was sensitive to PNGase F but resistant to Endo H, indicating a pattern consistent with complex rather than high mannose type glycosylation. When sorted by advancing tumor stage, peak expression of cathepsin B protein occurred in carcinomas involved in local invasion compared with adenomas or metastatic cancers. At all stages, cathepsin B activity correlated significantly with the levels of heavy chain mature cathepsin B protein (r = 0.6682, p <0.0001) irrespective of glycosylation. Immunohistochemical staining of cathepsin B protein revealed fine diffuse cytoplasmic staining in both adenomas and carcinomas compared with coarse granular cytoplasmic staining (typical of lysosomes) seen in matched normal mucosa. Our results demonstrate several sequential, apparently independent changes in cathepsin B expression during colorectal tumor progression including early changes in subcellular localization, up-regulation of cathepsin B protein and activity in invasive cancers, and altered protein glycosylation detected in malignant tumors at all stages.

Original languageEnglish (US)
Pages (from-to)29190-29199
Number of pages10
JournalJournal of Biological Chemistry
Volume272
Issue number46
DOIs
StatePublished - Nov 14 1997
Externally publishedYes

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Glycosylation
Cathepsin B
Enzyme activity
Tumors
Colorectal Neoplasms
Enzymes
Proteins
Adenoma
Carcinoma
Staining and Labeling
Neoplasms
Mucous Membrane
Western Blotting
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Enzyme Assays
Mannose
Lysosomes
Assays
Colon
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry

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Elevations in cathepsin B protein content and enzyme activity occur independently of glycosylation during colorectal tumor progression. / Iacobuzio-Donahue, Christine A.; Shuja, Sania; Cai, Jinguo; Peng, Phyllis; Murnane, Mary Jo.

In: Journal of Biological Chemistry, Vol. 272, No. 46, 14.11.1997, p. 29190-29199.

Research output: Contribution to journalArticle

Iacobuzio-Donahue, Christine A. ; Shuja, Sania ; Cai, Jinguo ; Peng, Phyllis ; Murnane, Mary Jo. / Elevations in cathepsin B protein content and enzyme activity occur independently of glycosylation during colorectal tumor progression. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 46. pp. 29190-29199.
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