Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

Uzma A. Chaudhry, Hean Zhuang, Barbara J. Crain, Sylvain Doré

Research output: Contribution to journalArticle

Abstract

Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

Original languageEnglish (US)
Pages (from-to)6-13
Number of pages8
JournalAlzheimer's and Dementia
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2008

Keywords

  • Cyclooxygenase
  • Middle frontal gyrus
  • Neuroinflammation
  • PGES
  • β-Amyloid

ASJC Scopus subject areas

  • Epidemiology
  • Health Policy
  • Developmental Neuroscience
  • Clinical Neurology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

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