Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

Uzma A. Chaudhry; Hean Zhuang; Barbara J. Crain; Sylvain Doré

doi:10.1016/j.jalz.2007.10.015

Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

Uzma A. Chaudhry, Hean Zhuang, Barbara J. Crain, Sylvain Doré

School of Medicine

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Background: The proinflammatory prostaglandin E₂ (PGE₂) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

Original language	English (US)
Pages (from-to)	6-13
Number of pages	8
Journal	Alzheimer's and Dementia
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.jalz.2007.10.015
State	Published - Jan 2008

Keywords

Cyclooxygenase
Middle frontal gyrus
Neuroinflammation
PGES
β-Amyloid

ASJC Scopus subject areas

Epidemiology
Health Policy
Developmental Neuroscience
Clinical Neurology
Geriatrics and Gerontology
Psychiatry and Mental health
Cellular and Molecular Neuroscience

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10.1016/j.jalz.2007.10.015

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title = "Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease",

abstract = "Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.",

keywords = "Cyclooxygenase, Middle frontal gyrus, Neuroinflammation, PGES, β-Amyloid",

author = "Chaudhry, {Uzma A.} and Hean Zhuang and Crain, {Barbara J.} and Sylvain Dor{\'e}",

note = "Funding Information: This work was supported by NIH grants AG022971 (S.D., B.C.) and NS046400 (S.D.). Postmortem brain tissues were provided by the Johns Hopkins University Alzheimer{\textquoteright}s Disease Research Center (AG05146). We thank Eduardo Zamora, MD for his technical assistance and all members of the Dor{\'e} lab for helpful discussions.",

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doi = "10.1016/j.jalz.2007.10.015",

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AU - Chaudhry, Uzma A.

AU - Zhuang, Hean

AU - Crain, Barbara J.

AU - Doré, Sylvain

N1 - Funding Information: This work was supported by NIH grants AG022971 (S.D., B.C.) and NS046400 (S.D.). Postmortem brain tissues were provided by the Johns Hopkins University Alzheimer’s Disease Research Center (AG05146). We thank Eduardo Zamora, MD for his technical assistance and all members of the Doré lab for helpful discussions.

PY - 2008/1

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N2 - Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

AB - Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

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Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

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Keywords

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