Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

Uzma A. Chaudhry, Hean Zhuang, Barbara J. Crain, Sylvain Doré

Research output: Contribution to journalArticle

Abstract

Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

Original languageEnglish (US)
Pages (from-to)6-13
Number of pages8
JournalAlzheimer's and Dementia
Volume4
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Alzheimer Disease
Astrocytes
Microglia
Fluorescent Antibody Technique
Endothelial Cells
Western Blotting
Neurons
Pyramidal Cells
Brain
Prostaglandin-E Synthases
Dinoprostone
Cerebrospinal Fluid
Immunohistochemistry
Staining and Labeling
Antibodies

Keywords

  • β-Amyloid
  • Cyclooxygenase
  • Middle frontal gyrus
  • Neuroinflammation
  • PGES

ASJC Scopus subject areas

  • Health Policy
  • Epidemiology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience
  • Clinical Neurology

Cite this

Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease. / Chaudhry, Uzma A.; Zhuang, Hean; Crain, Barbara J.; Doré, Sylvain.

In: Alzheimer's and Dementia, Vol. 4, No. 1, 01.2008, p. 6-13.

Research output: Contribution to journalArticle

Chaudhry, Uzma A. ; Zhuang, Hean ; Crain, Barbara J. ; Doré, Sylvain. / Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease. In: Alzheimer's and Dementia. 2008 ; Vol. 4, No. 1. pp. 6-13.
@article{f7533aed27b3463f893470c2f2acfb63,
title = "Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease",
abstract = "Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.",
keywords = "β-Amyloid, Cyclooxygenase, Middle frontal gyrus, Neuroinflammation, PGES",
author = "Chaudhry, {Uzma A.} and Hean Zhuang and Crain, {Barbara J.} and Sylvain Dor{\'e}",
year = "2008",
month = "1",
doi = "10.1016/j.jalz.2007.10.015",
language = "English (US)",
volume = "4",
pages = "6--13",
journal = "Alzheimer's and Dementia",
issn = "1552-5260",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

AU - Chaudhry, Uzma A.

AU - Zhuang, Hean

AU - Crain, Barbara J.

AU - Doré, Sylvain

PY - 2008/1

Y1 - 2008/1

N2 - Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

AB - Background: The proinflammatory prostaglandin E2 (PGE2) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains. Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies. Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls. Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

KW - β-Amyloid

KW - Cyclooxygenase

KW - Middle frontal gyrus

KW - Neuroinflammation

KW - PGES

UR - http://www.scopus.com/inward/record.url?scp=38049071474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38049071474&partnerID=8YFLogxK

U2 - 10.1016/j.jalz.2007.10.015

DO - 10.1016/j.jalz.2007.10.015

M3 - Article

C2 - 18631945

AN - SCOPUS:38049071474

VL - 4

SP - 6

EP - 13

JO - Alzheimer's and Dementia

JF - Alzheimer's and Dementia

SN - 1552-5260

IS - 1

ER -