Elevated microRNA miR-21 levels in pancreatic cyst fluid are predictive of mucinous precursor lesions of ductal adenocarcinoma

Ji Kon Ryu, Hanno Matthaei, Marco Dal Molin, Seung Mo Hong, Marcia Canto, Richard D. Schulick, Christopher Wolfgang, Michael S Goggins, Ralph H Hruban, Leslie Cope, Anirban Maitra

Research output: Contribution to journalArticle

Abstract

Background: Biomarkers for the diagnostic classification of pancreatic cysts are urgently needed. Deregulated microRNA (miRNAs) expression is widespread in pancreatic cancer. We assessed whether aberrant miRNAs in pancreatic cyst fluid could be used as potential biomarkers for cystic precursor lesions of pancreatic cancer. Methods: Cyst fluid specimens were prospectively collected from 40 surgically resected pancreatic cysts, and small RNAs were extracted. The 'mucinous' cohort included 14 intraductal papillary mucinous neoplasms (including 3 with an associated adenocarcinoma) and 10 mucinous cystic neoplasms; the 'nonmucinous' cohort included 11 serous cystadenomas and 5 other benign cysts. Quantitative reverse transcription PCR was performed for five miRNAs (miR-21, miR-155, miR-221, miR-17-3p, miR-191), which were previously reported as overexpressed in pancreatic adenocarcinomas. Results: Significantly higher expression of miR-21, miR-221, and miR-17-3p was observed in the mucinous versus nonmucinous cysts (p <0.01), with the mean relative fold differences being 7.0-, 7.9-, and 5.4-fold, respectively. Receiver operating characteristic curves demonstrated the highest median area under the curve for miR-21, with a median specificity of 76%, at a sensitivity of 80%. Conclusion: This pilot study demonstrates that profiling miRNAs in pancreatic cyst fluid samples is feasible and can yield potential biomarkers for the classification of cystic lesions of the pancreas.

Original languageEnglish (US)
Pages (from-to)343-350
Number of pages8
JournalPancreatology
Volume11
Issue number3
DOIs
StatePublished - Sep 2011

Fingerprint

Pancreatic Cyst
Cyst Fluid
MicroRNAs
Adenocarcinoma
Biomarkers
Pancreatic Neoplasms
Cysts
Serous Cystadenoma
Mucinous Adenocarcinoma
ROC Curve
Reverse Transcription
Area Under Curve
Pancreas
Neoplasms
RNA
Polymerase Chain Reaction

Keywords

  • Biomarker
  • Intraductal papillary mucinous neoplasm
  • microRNA
  • Mucinous cystic neoplasm
  • Pancreatic cyst

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Hepatology

Cite this

Elevated microRNA miR-21 levels in pancreatic cyst fluid are predictive of mucinous precursor lesions of ductal adenocarcinoma. / Ryu, Ji Kon; Matthaei, Hanno; Dal Molin, Marco; Hong, Seung Mo; Canto, Marcia; Schulick, Richard D.; Wolfgang, Christopher; Goggins, Michael S; Hruban, Ralph H; Cope, Leslie; Maitra, Anirban.

In: Pancreatology, Vol. 11, No. 3, 09.2011, p. 343-350.

Research output: Contribution to journalArticle

@article{41dc3649eb6b4b2b8efb48be1293a230,
title = "Elevated microRNA miR-21 levels in pancreatic cyst fluid are predictive of mucinous precursor lesions of ductal adenocarcinoma",
abstract = "Background: Biomarkers for the diagnostic classification of pancreatic cysts are urgently needed. Deregulated microRNA (miRNAs) expression is widespread in pancreatic cancer. We assessed whether aberrant miRNAs in pancreatic cyst fluid could be used as potential biomarkers for cystic precursor lesions of pancreatic cancer. Methods: Cyst fluid specimens were prospectively collected from 40 surgically resected pancreatic cysts, and small RNAs were extracted. The 'mucinous' cohort included 14 intraductal papillary mucinous neoplasms (including 3 with an associated adenocarcinoma) and 10 mucinous cystic neoplasms; the 'nonmucinous' cohort included 11 serous cystadenomas and 5 other benign cysts. Quantitative reverse transcription PCR was performed for five miRNAs (miR-21, miR-155, miR-221, miR-17-3p, miR-191), which were previously reported as overexpressed in pancreatic adenocarcinomas. Results: Significantly higher expression of miR-21, miR-221, and miR-17-3p was observed in the mucinous versus nonmucinous cysts (p <0.01), with the mean relative fold differences being 7.0-, 7.9-, and 5.4-fold, respectively. Receiver operating characteristic curves demonstrated the highest median area under the curve for miR-21, with a median specificity of 76{\%}, at a sensitivity of 80{\%}. Conclusion: This pilot study demonstrates that profiling miRNAs in pancreatic cyst fluid samples is feasible and can yield potential biomarkers for the classification of cystic lesions of the pancreas.",
keywords = "Biomarker, Intraductal papillary mucinous neoplasm, microRNA, Mucinous cystic neoplasm, Pancreatic cyst",
author = "Ryu, {Ji Kon} and Hanno Matthaei and {Dal Molin}, Marco and Hong, {Seung Mo} and Marcia Canto and Schulick, {Richard D.} and Christopher Wolfgang and Goggins, {Michael S} and Hruban, {Ralph H} and Leslie Cope and Anirban Maitra",
year = "2011",
month = "9",
doi = "10.1159/000329183",
language = "English (US)",
volume = "11",
pages = "343--350",
journal = "Pancreatology",
issn = "1424-3903",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Elevated microRNA miR-21 levels in pancreatic cyst fluid are predictive of mucinous precursor lesions of ductal adenocarcinoma

AU - Ryu, Ji Kon

AU - Matthaei, Hanno

AU - Dal Molin, Marco

AU - Hong, Seung Mo

AU - Canto, Marcia

AU - Schulick, Richard D.

AU - Wolfgang, Christopher

AU - Goggins, Michael S

AU - Hruban, Ralph H

AU - Cope, Leslie

AU - Maitra, Anirban

PY - 2011/9

Y1 - 2011/9

N2 - Background: Biomarkers for the diagnostic classification of pancreatic cysts are urgently needed. Deregulated microRNA (miRNAs) expression is widespread in pancreatic cancer. We assessed whether aberrant miRNAs in pancreatic cyst fluid could be used as potential biomarkers for cystic precursor lesions of pancreatic cancer. Methods: Cyst fluid specimens were prospectively collected from 40 surgically resected pancreatic cysts, and small RNAs were extracted. The 'mucinous' cohort included 14 intraductal papillary mucinous neoplasms (including 3 with an associated adenocarcinoma) and 10 mucinous cystic neoplasms; the 'nonmucinous' cohort included 11 serous cystadenomas and 5 other benign cysts. Quantitative reverse transcription PCR was performed for five miRNAs (miR-21, miR-155, miR-221, miR-17-3p, miR-191), which were previously reported as overexpressed in pancreatic adenocarcinomas. Results: Significantly higher expression of miR-21, miR-221, and miR-17-3p was observed in the mucinous versus nonmucinous cysts (p <0.01), with the mean relative fold differences being 7.0-, 7.9-, and 5.4-fold, respectively. Receiver operating characteristic curves demonstrated the highest median area under the curve for miR-21, with a median specificity of 76%, at a sensitivity of 80%. Conclusion: This pilot study demonstrates that profiling miRNAs in pancreatic cyst fluid samples is feasible and can yield potential biomarkers for the classification of cystic lesions of the pancreas.

AB - Background: Biomarkers for the diagnostic classification of pancreatic cysts are urgently needed. Deregulated microRNA (miRNAs) expression is widespread in pancreatic cancer. We assessed whether aberrant miRNAs in pancreatic cyst fluid could be used as potential biomarkers for cystic precursor lesions of pancreatic cancer. Methods: Cyst fluid specimens were prospectively collected from 40 surgically resected pancreatic cysts, and small RNAs were extracted. The 'mucinous' cohort included 14 intraductal papillary mucinous neoplasms (including 3 with an associated adenocarcinoma) and 10 mucinous cystic neoplasms; the 'nonmucinous' cohort included 11 serous cystadenomas and 5 other benign cysts. Quantitative reverse transcription PCR was performed for five miRNAs (miR-21, miR-155, miR-221, miR-17-3p, miR-191), which were previously reported as overexpressed in pancreatic adenocarcinomas. Results: Significantly higher expression of miR-21, miR-221, and miR-17-3p was observed in the mucinous versus nonmucinous cysts (p <0.01), with the mean relative fold differences being 7.0-, 7.9-, and 5.4-fold, respectively. Receiver operating characteristic curves demonstrated the highest median area under the curve for miR-21, with a median specificity of 76%, at a sensitivity of 80%. Conclusion: This pilot study demonstrates that profiling miRNAs in pancreatic cyst fluid samples is feasible and can yield potential biomarkers for the classification of cystic lesions of the pancreas.

KW - Biomarker

KW - Intraductal papillary mucinous neoplasm

KW - microRNA

KW - Mucinous cystic neoplasm

KW - Pancreatic cyst

UR - http://www.scopus.com/inward/record.url?scp=79960248965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960248965&partnerID=8YFLogxK

U2 - 10.1159/000329183

DO - 10.1159/000329183

M3 - Article

C2 - 21757972

AN - SCOPUS:79960248965

VL - 11

SP - 343

EP - 350

JO - Pancreatology

JF - Pancreatology

SN - 1424-3903

IS - 3

ER -