Electroporation-mediated gene transfer in cardiac tissue

Rachel L. Harrison, Barry J. Byrne, Leslie Tung

Research output: Contribution to journalArticlepeer-review

Abstract

Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks. GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalFEBS Letters
Volume435
Issue number1
DOIs
StatePublished - Sep 11 1998

Keywords

  • Electroporation
  • Embryonic chick heart
  • Gene transfer
  • Green fluorescent protein
  • Luciferase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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