Electroporation-mediated gene transfer in cardiac tissue

Rachel L. Harrison; Barry J. Byrne; Leslie Tung

doi:10.1016/S0014-5793(98)00987-9

Electroporation-mediated gene transfer in cardiac tissue

Rachel L. Harrison, Barry J. Byrne, Leslie Tung

School of Medicine

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks. GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	FEBS Letters
Volume	435
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(98)00987-9
State	Published - Sep 11 1998

Keywords

Electroporation
Embryonic chick heart
Gene transfer
Green fluorescent protein
Luciferase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00987-9

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title = "Electroporation-mediated gene transfer in cardiac tissue",

abstract = "Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks. GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes. Copyright (C) 1998 Federation of European Biochemical Societies.",

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AB - Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks. GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes. Copyright (C) 1998 Federation of European Biochemical Societies.

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KW - Luciferase

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Electroporation-mediated gene transfer in cardiac tissue

Abstract

Keywords

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